Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

SAGETRACK

Sign In to gain access to subscriptions and/or personal tools.
Journal of the Society for Gynecologic Investigation
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Pollard, J. K.
Right arrow Articles by Mitchell, M. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pollard, J. K.
Right arrow Articles by Thai, D.
Right arrow Articles by Mitchell, M. D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Mechanism of Cytokine Stimulation of Prostaglandin Biosynthesis in Human Decidua

Jeffrey K. Pollard

Department of Obstetrics and Gynecology, University of Utah School of Medicine, Salt Lake City, Utah

Dave Thai

Department of Obstetrics and Gynecology, University of Utah School of Medicine, Salt Lake City, Utah

Murray D. Mitchell

Department of Obstetrics and Gynecology, University of Utah School of Medicine, Salt Lake City, Utah

OBJECTIVE: The purpose of this study was to determine the mechanism of action of the cytokines tumor necrosis factor-{alpha} (TNF{alpha}) and interleukin-1ß (IL-1ß) on the stimulation of prostaglandin (PG) production in human decidua.

METHODS: Decidual cells from term placentas were grown in culture until confluent. Incuba tions were performed with TNF{alpha} or IL-1ß, and with cycloheximide, actinomycin D, arachidonic acid, or acetylsalicylic acid (ASA). Prostaglandin E2 was measured by radioimmunoassay and cellular protein determined.

RESULTS: The concentration-related stimulation of decidual PGE2 production by IL-1ß and TNF{alpha} was completely abrogated by cycloheximide and actinomycin D treatment. Although arachidonic acid alone stimulated decidual PGE2 biosynthesis, the addition of IL-1ß or TNF{alpha} consistently augmented this effect. Both cytokines induced recovery of PGE2 biosynthesis from ASA pretreatment more rapidly than controls.

CONCLUSIONS: Interleukin-1ß and TNF{alpha} both act on decidual PG biosynthesis in a manner requiring new protein synthesis. In combination with our other results, this suggests that IL-1ß and TNF{alpha} act to induce PG endoperoxide synthase activity. (J Soc Gynecol Invest 1994;1: 31-6)

Key Words: Prostaglandins • preterm labor • cytokines • decidua.

Journal of the Society for Gynecologic Investigation, Vol. 1, No. 1, 31-36 (1994)
DOI: 10.1177/107155769400100107
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?