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Journal of the Society for Gynecologic Investigation
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Mechanism of Cytokine Stimulation of Prostaglandin Biosynthesis in Human Decidua

Jeffrey K. Pollard

Department of Obstetrics and Gynecology, University of Utah School of Medicine, Salt Lake City, Utah

Dave Thai

Department of Obstetrics and Gynecology, University of Utah School of Medicine, Salt Lake City, Utah

Murray D. Mitchell

Department of Obstetrics and Gynecology, University of Utah School of Medicine, Salt Lake City, Utah

OBJECTIVE: The purpose of this study was to determine the mechanism of action of the cytokines tumor necrosis factor-{alpha} (TNF{alpha}) and interleukin-1ß (IL-1ß) on the stimulation of prostaglandin (PG) production in human decidua.

METHODS: Decidual cells from term placentas were grown in culture until confluent. Incuba tions were performed with TNF{alpha} or IL-1ß, and with cycloheximide, actinomycin D, arachidonic acid, or acetylsalicylic acid (ASA). Prostaglandin E2 was measured by radioimmunoassay and cellular protein determined.

RESULTS: The concentration-related stimulation of decidual PGE2 production by IL-1ß and TNF{alpha} was completely abrogated by cycloheximide and actinomycin D treatment. Although arachidonic acid alone stimulated decidual PGE2 biosynthesis, the addition of IL-1ß or TNF{alpha} consistently augmented this effect. Both cytokines induced recovery of PGE2 biosynthesis from ASA pretreatment more rapidly than controls.

CONCLUSIONS: Interleukin-1ß and TNF{alpha} both act on decidual PG biosynthesis in a manner requiring new protein synthesis. In combination with our other results, this suggests that IL-1ß and TNF{alpha} act to induce PG endoperoxide synthase activity. (J Soc Gynecol Invest 1994;1: 31-6)

Key Words: Prostaglandins • preterm labor • cytokines • decidua.

Journal of the Society for Gynecologic Investigation, Vol. 1, No. 1, 31-36 (1994)
DOI: 10.1177/107155769400100107
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