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Modulators of Prostaglandin E2 Synthesis in Human Amnion

Department of Obstetrics and Gynecology, University of South Florida College of Medicine, Tampa, Florida

William F. O'Brien, MD

Department of Obstetrics and Gynecology, University of South Florida College of Medicine, Tampa, Florida

Mark C. Williams, MD

Department of Obstetrics and Gynecology, University of South Florida College of Medicine, Tampa, Florida

OBJECTIVE: The purpose of these studies was to determine the effects of the essential fatty acid, linoleic acid, and the commonly used non-steroidal anti-inflammatory agents, aspirin and acet aminophen, on the rate of prostaglandin (PG) biosynthesis by human amnion cells.

METHODS: Amnion cells were isolated from term, normal pregnancies and grown to confluence. Cells were incubated with control or medium containing 100 µ mol/L linoleic acid. Cells were also incubated with control medium or medium containing 10 or 100 µg/mL aspirin or acetaminophen.

RESULTS: Following an initial delay, amnion cells exposed to linoleic acid exhibited a signif icant increase in PGE synthesis. Both aspirin and acetaminophen in clinically relevant concen trations had a significant inhibitory effect on amnion cell PGE synthesis.

CONCLUSIONS: Linoleic acid has a stimulatory effect and aspirin and acetaminophen have an inhibitory effect on PGE synthesis in human amnion cells in culture. We speculate that dietary habits, supplement ingestion, and over-the-counter drug use may affect amnion cell PG production. In view of the potential importance of intrauterine PG production in normal and abnormal labor, further study in this area is indicated. (J Soc Gynecol Invest 1994;1:131-4)

Key Words: Prostaglandin • amnion • acetaminophen • aspirin.

Journal of the Society for Gynecologic Investigation, Vol. 1, No. 2, 131-134 (1994)
DOI: 10.1177/107155769400100206


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