Journal of the Society for Gynecologic Investigation

 

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Journal of the Society for Gynecologic Investigation, Vol. 1, No. 4, 302-309 (1994)
DOI: 10.1177/107155769400100410

The Anabolic Effects of Progesterone on Fetal Rat Calvaria in Tissue Culture

Deborah L. Manzi

Carol C. Pilbeam

Lawrence G. Raisz

OBJECTIVE: We examined the effects of progesterone and cortisol on [3H] thymidine incorpo ration into DNA, total DNA content, and [3H] proline incorporation into collagen using a bone organ culture system.

METHODS: The study consisted of nine experiments conducted at the University of Connecticut after approval by the animal care committee. Fetal rat half calvaria were cultured in the presence of progesterone (10-5 to 10-15 mol/L) and/or cortisol (10-7 mol/L). Statistical analysis was carried out by analysis of variance.

RESULTS: There was a biphasic increase in [3H] thymidine incorporation and DNA content of the tissue sample with progesterone treatment, with peaks at both high and low concentrations. Significant increases in [3H] proline incorporation were noted at concentrations of 10-6 to 10-10 mol/L of progesterone. Similar responses were observed in the presence of cortisol.

CONCLUSIONS: Progesterone increased [3H] thymidine incorporation into DNA, total DNA content, and [3H] proline incorporation into collagen proteins in cultured fetal rat calvaria over a wide range of progesterone concentrations in the presence and absence of cortisol. (J Soc Gynecol Invest 1994;1:302-9)

Key Words: Osteoporosis • progesterone • rat calvaria.


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