Journal of the Society for Gynecologic Investigation

 

Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

http://mc.manuscriptcentral.com/rsci

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rychlik, D. F.
Right arrow Articles by Phillippe, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rychlik, D. F.
Right arrow Articles by Chien, E. K.
Right arrow Articles by Wolff, D.
Right arrow Articles by Phillippe, S.
Right arrow Articles by Phillippe, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of the Society for Gynecologic Investigation, Vol. 10, No. 2, 67-73 (2003)
DOI: 10.1016/s1071-55760200252-6

Cloning and Tissue expression of the Tissue Prothrombinase Fgl-2 in the Sprague-Dawley Rat

Daniel F. Rychlik, MD

Edward K. Chien, MD

David Wolff, MS

Shiela Phillippe, MLIS

Mark Phillippe, MD

Rinehard Center for Reproductive Medicinem Evanston, Illinois; University of Vermont, Burlington, Vermont; Northwestern University, Evanston, Illinois

Objective: To sequence and characterize the expression of the prothrombinase Fgl-2 in the Sprague-Dawley rat.

Methods: Reverse-transcriptase polymerase chain reaction was performed on RNA from spontaneoulsy cycling adult pregnant Sprague-Dawley rats by using specific Fgl-w primers. The resulting amplicon was also used to screen a rat spleen bacteriophage library and to probe a Northern blot of various tissues. The rat Fgl-2 amino acid sequence was compared with the known sequences in mouse and human.

Results: Fgl-w specific amplicon bands were observed in the rat brain, kidney, liver, ovary, spleen, and gestational day 22 and postpartum uterus. The rat Fgl-2 cDNA and amino acid sequence were found to be homologous with those of human (86% and 74%, respectively) and mouse (91% and 87%, respectively). Northern blotting demonstrated two different-sized transcripts (1.3 and 3.4 kb), and expression was observed in the cevix, heart, liver, ovary, and nongestational and gestational day 22 myometrium.

Conclusion: Thrombin is classically generated from the clevage of the proenzyme prothrombin by activated factors V and X. In tissues thrombin appears to be generated by a novel prothrombinase Fgl-2 (fibrinogen-like protein) whose activity is stimulated by proinflammatory mediators. Fgl-2 provides the mechanistic coupling between proinflammatory cytokines and generation of active thrombin independent of the coagulation cascade. Our studies confirmed the expression of Fgl-2 mRNA in several rat tissues, including the pregnant uterus, where it could play a key role in the initiation of parturition especially in response to local or systemic infection.

Key Words: Prothrombinase • Fgl-2 • parturition


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?