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EP3-2 Receptor mRNA Expression Is Reduced and EP3-6 Receptor mRAN Expression Is Increased in Gravid Human Myometrium

Departments of Obstetrics and Gynecology and Pathology, Keck School of Medicine, University of Southern California, Los Angeles, California; Women's and Children's Hospital, Los Angeles County + University of Southern California Medical Center, 1240 North Mission Road, Room 5K40, Los Angeles California 90033; dwing{at}hsc.usc.edu

Nima Goharkhay, MD

Mary Hanna, MD

Yathi M. Naidu, PhD

Bruce W. Kovacs, MD

Juan C. Felix, MD

Departments of Obstetrics and Gynecology and Pathology, Keck School of Medicine, University of Southern California, Los Angeles, California

Objective: To assess relative expression levels of mRNA for EP3 isoforms EP3-2, EP3-3, and EP3-6 in human myometrium in various physiologic states.

Methods: Using semiquantitative reverse transcriptase-polymerase chain reaction, we analyzed myometrial samples from 46 women (ten menopausal, ten nongravid premenopausal, 19 gravidas, seven premenopausal misoprostol-treated nongravid women) for the specific expression of mRNA messages for the EP3 receptor isoforms EP3-2, EP3-3, and EP3-6.

Results: We found that the expression of EP3-2 was significantly lower in gravid than nongravid myometrium (P < .0001). Levels of the EP3-6 message were higher in gravid samples than in nongravid specimens (P < .02). EP3-3 mRNA was present in higher levels in menopausal samples compared with the other groups.

Conclusions: The selective fluctuation of mRNA expression of the different EP3 isoforms suggests the possibility of an important regulatory role for these receptors in myometrial contractility. Additional studies are necessary to evaluate the exact mechanism of action of EP3 receptor isoforms in human myometrium.

Key Words: EP3 receptor isoforms • mRNA expression • human myometrium • prostaglandin E2

Journal of the Society for Gynecologic Investigation, Vol. 10, No. 3, 124-129 (2003)
DOI: 10.1016/S1071-55760300007-8


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