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Effects of Multiple Courses of Antenatal Corticosteroids on Regional Brain and Somatic Tissue Water Content in Ovine Fetuses

Department of Pediatrics, Brown University Medical School, Women and Infants' Hospital of Rhode Island, Providence, Rhode Island; BStonest{at}wihri.org

Shannon Watkins

Katherine H. Petersson, PhD

Grazyna B. Sadowska, DVM

Department of Pediatrics, Brown University Medical School, Women and Infants' Hospital of Rhode Island, Providence, Rhode Island

Objective: To study the effects of single and multiple courses of antenatal corticosteroids on tissue water content in ovine fetuses.

Methods: After chronic catheterization of the ewes and fetuses, the ewes were randomly assigned to receive single or multiple courses of dexamethasone or placebo beginning at 104-106 or 76-78 days' gestation, respectively. In the single course groups, the ewes received dexamethansone (6 mg, n = 6) or placebo (n = 6) as four intramuscular injections every 12 hours over 48 hours. The fetal tissues were harvested for water content determination 66 hours after the first injection of dexamethasone or placebo was given. In the multiple-course groups, the ewes received the same treatment (dexamethasone, n = 10, or placebo, n = 8), once a week for 5 weeks starting at 76-78 days' gestation. In these groups, the tissues were harvested 66 hours after the first the injection of the fifth and last treatment course. In both groups, tissues were harvested at 106-107 days' gestation. Tissue water content was determined by wet-to-dry weight ratio in brain (cerebral cortex, caudate nucleus, cerebellum, midbrain, and medulla) and somatic tissues (kidney, liver, muscle, and skin).

Results: Water content in the brain regions (cerebellum and medulla) was lower (P < .05) in fetuses of dexamethasone-treated ewes than placebo-treated ewes than placebo-treated ewes after the multiple courses, but not the single course. Water content of somatic tissue was power (P < .05) in fetuses of dexamethasone-treated ewes than placebo-treated ewes after the multiple courses, and in the liver after a single course.

Conclusion: Dexamethasone treatment of ewes at 70% of gestation results in decreased regional brain water content in the fetuses after multiple but not single treatment courses, in somatic tissues (kidney, liver, muscle, and skin) after multiple courses, and in the liver after a single course.

Key Words: Brain • dexamethasone • fetus • multiple-course steroids • ovine • sheep • water content

Journal of the Society for Gynecologic Investigation, Vol. 11, No. 3, 166-174 (2004)
DOI: 10.1016/j.jsgi.2003.10.006


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