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Induction of Insulin-Like Growth Factor Binding Protein-1 Expression in Baboon Endometrial Stromal Cells by Cells of Trophoblast Origin

V. Han, MD

Departments of Obstetrics and Gynecology and Physiology and Biophysics, University of Illinois at Chicago, Chicago, Illinois; Departments of Obstetrics and Gynecology and Pediatrics, CIHR Group in Fetal and Neonatal Health and Development, The Child Health Research Institute, The Lawson Health Research Institute, and The University of Western Ontario, London, Ontario, Canada

A. T. Fazleabas, PhD

Department of Obstetrics and Gynecology, University of Illinois at Chicago, 820 S. Wood St, M/C 808, Chicago, IL 60612 asgi{at}uic.edu

J. Julie Kim, PhD

Departments of Obstetrics and Gynecology and Physiology and Biophysics, University of Illinois at Chicago, Chicago, Illinois; Departments of Obstetrics and Gynecology and Pediatrics, CIHR Group in Fetal and Neonatal Health and Development, The Child Health Research Institute, The Lawson Health Research Institute, and The University of Western Ontario, London, Ontario, Canada

Objective: To determine the influence of the conceptus on the induction of decidualization in endometrial stromal cells from the baboon.

Methods: For in vivo studies, implantation sites from day 22 of pregnancy in the baboon were analyzed for insulin-like growth factor-II (IGF-II) and insulin-like growth factor binding protein-1 (IGFBP-1) mRNAs using in situ hybridization. For in vitro studies, Jeg-3 cells or primary cytotrophoblasts isolated from term placenta were cocultured with a monolayer of stromal cells from the baboon endometrium. Cytotrophoblasts were placed either directly on top of the stromal cell monolayer or in transwell cell culture inserts and cultured for 7 days. No exogenous hormones were added. Stromal cells were analyzed for IGFBP-1 mRNA and protein by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunocytochemistry.

Results: Examination of early implantation sites in the baboon revealed a high expression of IGF-II mRNA in the invading cytotrophoblasts. Conversely, the stromal cells of the endometrium directly adjacent to the cytotrophoblasts expressed IGFBP-1 mRNA. Endometrial stromal cells cocultured with Jeg-3 cells or primary cytotrophoblasts for 7 days expressed IGFBP-1 mRNA and protein. This expression occurred in both the direct coculture system and coculture using the cell culture inserts. Cytotrophoblasts also induced the expression of prolactin in stromal fibroblasts following coculture.

Conclusions: The concepts is capable of inducing decidualization of endometrial stromal cells. This is shown in both the in vivo and in vitro systems.

Key Words: Decidualization • IGF • pregnancy • cytotrophoblast • endometrium

Journal of the Society for Gynecologic Investigation, Vol. 11, No. 6, 399-405 (2004)
DOI: 10.1016/j.jsgi.2004.03.005


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