Journal of the Society for Gynecologic Investigation

 

Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

http://mc.manuscriptcentral.com/rsci

SAGETRACK

Sign In to gain access to subscriptions and/or personal tools.
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gielen, S. C.J.P.
Right arrow Articles by Burger, C. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of the Society for Gynecologic Investigation, Vol. 12, No. 1, 58-64 (2005)
DOI: 10.1016/j.jsgi.2004.08.003

Steroid-Modulated Proliferation of Human Endometrial Carcinoma Cell Lines: Any Role for Insulin-like Growth Factor Signaling?

Susanne C.J.P. Gielen, MD

Department of Obstetrics and Gynecology and the Department of Reproduction and Development, Erasmus Medical Center, Rotterdam; Department of Obstetrics and Gynecology, Ruwaard van Putten Hospital, Spijkenisse, The Netherlands s.gielen{at}erasmusmc.nl

Eline E. Hanekamp, MSc

Leen J. Blok, PhD

Frans J. Huikeshoven, MD, PhD

Curt W. Burger, MD, PhD

Department of Obstetrics and Gynecology and the Department of Reproduction and Development, Erasmus Medical Center, Rotterdam; Department of Obstetrics and Gynecology, Ruwaard van Putten Hospital, Spijkenisse, The Netherlands

Objectives: Estrogen-stimulated proliferation of the normal and malignant human endometrium is balanced by the differentiating properties ofprogesterone. This study evaluated the role of insulin-like growth factor (IGF) signaling in steroid-induced modulation of endometrial cancer cell proliferation.

Methods: We used the human endometrial, estrogen-responsive ECC-1 and progesterone-responsive PRAB-36 cell lines. Proliferation studies with IGFs in combination with either estrogen orprogesterone were conducted. Furthermore, the mRNA and protein expression of insulin-like growth factor—binding proteins (IGFBPs) was evaluated.

Results: Using the ECC-1 cell line, we observed that estrogen-induced proliferation is modulated via the IGF-receptor signaling pathway, and that IGF-1-induced stimulation of prolferation does not influence estrogen receptor signaling. Furthermore, expression of the main modulators of IGF action, the IGFBPs, was found to be regulated by estrogen and progesterone in both cell lines. IGFBP-4 was up-regulated by estrogen in the ECC-1 cell line, and IGFBP-3 and IGFBP-6 were down-regulated by progesterone in the PRAB-36 cell line.

Conclusion: Estrogen-induced stimulation of prolferation of ECC-I endometrial cancer cells is partly achieved via IGF signaling. Furthermore, the IGFBPs are regulated by estrogens as well as progestagens and could potentially play a role in the modulation of endometrial cancer cell proliferation.

Key Words: Estrogen • progesterone • endometrium • proliferation regulation • IGF signaling • IGFBP


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?