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Mineralocorticoid Receptor Expression in Late-Gestation Ovine Fetal Lung

Department of Pharmnacodynamics, College of Pharmacy, and Department of Physiology and Functional Genomics, College of Medicine, University of Flonda, Gainesville, Flonda; Department of Pharmacodvnamics, Box 100487, University of Flonda, Gainesville, FL 32610-0487; kellerwood{at}cop.ufl.edu

Charles E. Wood, PhD

Yi Hua, MD, PhD

Daying Zhang, MD, PhD

Department of Pharmnacodynamics, College of Pharmacy, and Department of Physiology and Functional Genomics, College of Medicine, University of Flonda, Gainesville, Flonda.

Changes in adrenal corticosteroid secretion result in changes in lung liquid production in the late-gestation fetus. To testfor the presence of mineralocorticoid receptor (MR) in fital pulmonary epithelium, lungsfrom fetal sheep of 120 to 130 days'gestation (term about 148 days) were collected andfrozenfor identfication of mRNA for MR in homogenates by reverse transcriptase polymerase chain reaction (RT-PCR) orfor determination of ³H-cortisol binding at MR. Other samples offetal lungs werefixedfor localization of MR and Na,⁺, K⁺ adenosine triphosphatase (ATPase) by immunohistochemistry. MR mRNA was identified in lung tissuefromfetuses and newborn lambs, but notfrom pregnant ewes; MR-regulated genes, including SGK1 and ENaC were also expressed infital and newborn lungs. Immunoreactive MR was found in pulmonary epithelial cells and to be colocalized with Na⁺, K⁺ ATPase in many sites. These results indicate that the molecular apparatus for mineralocorticoid-stimulated lung liquid reabsorption is present in epithelium by 120 day's gestation.

Key Words: Corticosteroid receptors • MR • epithelial sodium channel • Na+ • K+ATPase

Journal of the Society for Gynecologic Investigation, Vol. 12, No. 2, 84-91 (2005)
DOI: 10.1016/j.jsgi.2004.10.010


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