This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Chen, H. Articles by Chen, X. Search for Related Content PubMed PubMed Citation Articles by Chen, H. Articles by Ye, D. Articles by Xie, X. Articles by Lu, W. Articles by Zhu, C. Articles by Chen, X. Pubmed/NCBI databases Gene GEO Profiles HomoloGene UniGene Genetics Home Reference Medline Plus Health Information High Risk Pregnancy Social Bookmarking                   What's this?

PTEN Promoter Methylation and Protein Expression in Normal Early Placentas and Hydatidiform Moles

Dafeng Ye, MD

Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China

Xing Xie, MD

Oncologic Department, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China; xiex{at}mail.hz.zj.cn

Weiguo Lu, MD

Changkun Zhu, MD

Xiaodong Chen, MD

Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China

Objective: To investigate the relationship between PTEN promoter methylation and protein expression, and the possible involvement of the PTEN gene in development of gestational trophoblasts and the pathogenesis of hydatidjform moles.

Methods: DNA was extracted from choria of normal early placentas, partial hydatidtform moles, complete hydatidiform moles, and invasive moles, and overdigested by methylation-sensitive endonuclease HpaII. The PTEN promoter was amplifcated by polymerase chain reaction. PTEN protein expression was detected by immunohistochemistry.

Results: In partial and complete hydatidiform moles, the PTEN promoter methylation rate was significantly higher than in early placentas (72%, 59.4%, 14.3%; P = .000, .002, respectively), and the PTEN protein expression rate was significantly lower than in early placentas (9.1%, 4.5%, 90.5%; P = .000, .000, respectively). However, partial hydatidiform moles, complete hydatidiform moles, and invasive moles were not significant different in terms of PTEN promoter methylation and protein expression.

Conclusions: These findings suggest that the regulation of PTEN expression may play an important role in the development of the early gestational trophoblast and in the pathogenesis of hydatidiform mole, but not in its malignant transformation.

Key Words: Trophoblasts • gestational trophoblastic disease • PTEN • promoter methylation

Journal of the Society for Gynecologic Investigation, Vol. 12, No. 3, 214-217 (2005)
DOI: 10.1016/j.jsgi.2005.01.009


CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?