This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Goumenou, A. Articles by Arici, A. Search for Related Content PubMed PubMed Citation Articles by Goumenou, A. Articles by Panayiotides, I. Articles by Mahutte, N. G. Articles by Matalliotakis, I. Articles by Fragouli, Y. Articles by Arici, A. Pubmed/NCBI databases Gene GEO Profiles HomoloGene UniGene Substance via MeSH Medline Plus Health Information Endometriosis Genetics Home Reference Social Bookmarking                         What's this?

Immunohistochemical Expression of p53, MDM2, and p21Wafi Oncoproteins in Endometriomas But Not Adenomyosis

Department of Obstetncs and Gynecology, University Hospital of Heraklion, Crete; Department of Pathology and Department of Hygiene and Epidemiology, University of Athens, Athens, Greece; Division of Reproductive Endocnnology and Infertility, Department of Obstetnrcs and Gynecology, Dartmouth Medical School, Lebanon, New Hampshire; Department of Obstetncs and Gynecology, Yale University School of Medicine, New Haven, Connecticut

Ioannis Panayiotides, MD, PhD

Neal G. Mahutte, MD

Department of Obstetncs and Gynecology, Yale University School of Medicine, New Haven, Connecticut; Department of Obstetrics and Gynecology, Dartmouth Medical School, One Medical Center Dr., Lebanon, NH 03756; Neal.G.Mahutte{at}Hitchcock.org

Ioannis Matalliotakis, MD, PhD

Yvoni Fragouli, MD, PhD

Aydin Arici, MD

Department of Obstetncs and Gynecology, University Hospital of Heraklion, Crete; Department of Pathology and Department of Hygiene and Epidemiology, University of Athens, Athens, Greece; Division of Reproductive Endocnnology and Infertility, Department of Obstetnrcs and Gynecology, Dartmouth Medical School, Lebanon, New Hampshire; Department of Obstetncs and Gynecology, Yale University School of Medicine, New Haven, Connecticut

Objective: p53, MDM2, and p21^Wafi are oncoproteins that regulate the cell cycle. The purpose of this study was to examine the distribution ofp53, MDM2, and p21 Wafl oncoprotein expression in endometriomas and in adenomyosis.

Methods: Tissue samples from 25 women with pathologically confirmed endometriomas and 31 women with pathologically confirmed adenomyosis were analyzed. Expression of p53, MDM2, and p21^Waf1 oncoproteins was assessed by immunohistochemical nuclear staining.

Results: p53, MDM2, and p21^Waf1 expression were detected in 20%, 60%, and 80% of endometrioma tissue samples, respectively. All endometrioma tissue samples expressing p53 also tested positive for both MDM2 and p21^Waf1 MDM2 expression was signnficantly higher in the proliferative than in the secretory phase of the cycle. In contrast,all 31 adenomyosis tissue samples were negativeforp53, MDM2, and p²¹ Wafl expression.

Conclusion: The expression of p53, MDM2, and p2J^Waf1 suggests a role for these oncoproteins in the regulation of endometrioma cell growth, but not in adenomyosis.

Key Words: Adenomyosis • endometriosis • immunohistochemistry • oncoprotein

Journal of the Society for Gynecologic Investigation, Vol. 12, No. 4, 263-266 (2005)
DOI: 10.1016/j.jsgi.2005.01.028


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?