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Hypoxia Up-regulated Angiogenin and Down-regulated Vascular Cell Adhesion Molecule-i Expression and Secretion in Human Placental Trophoblasts

Department of Obstetncs and Gynaecology and the Department of Paediatrics, National University Hospital, National University of Singapore, Singapore

A. Loganath, PhD

Department of Obstetncs and Gynaecology and the Department of Paediatrics, National University Hospital, National University of Singapore, Singapore obgannam{at}nus.edu.sg

A. C. Roy, PhD

S. S. Chong, PhD

Y. C. Wong, FRCOG

Department of Obstetncs and Gynaecology and the Department of Paediatrics, National University Hospital, National University of Singapore, Singapore

Objective: Many processes that are involved in cellular invasion, including blastocyst implantation, placental development, and rapidly growing tumors, occur in reduced oxygen environments. It has been surmised that oxygen tension could regulate the cytotrophoblast ability to differentiate and, as a consequence, to express proteins that are critical for placentation. The objective of the current investigation was therefore to test the hypothesis that placental tissues and trophoblast cells in culture, under low oxygen tension, release angiogenic factors that could affect vascular behavior and invasive potential, thus providing a link between abnormal placentation and maternal vascular abnormality.

Methods: Functionally active term placental explant culture and trophoblast cultures were used to demonstrate the secretion profiles of angiogenin and vascular cell adhesion molecule-1 (VCAM-1), and the real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) technique was employed to demonstrate the mRNA expression under both normoxic and hypoxic conditions.

Results: A significant increase in the secretion (P <.01) and mRNA expression (P <.01) of angiogenin and a signficant decrease in the secretion (P <.04) and mRNA expression (P <.03) of VCAM-1 from both term placental explants and trophoblast cultures subjected to hypoxia in vitro were observed.

Conclusion: Because the primary defect in uteroplacental insufficiency is placental maldevelopment probably associated with hypoxia in situ, this study provides molecular evidence to indicate that the differential expression and secretion of angiogenic factors may play an important role in these pathologic conditions.

Key Words: Angiogenesis • explant culture • fetal growth restriction • lightcycler • pregnancy

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