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Journal of the Society for Gynecologic Investigation
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The Effect of Serum From Women With Preeclampsia on JAR (trophoblast-like) Cell Line

Safa Mahameed, MD

Shlomit Goldman, DSc

Diane Gabarin, MD

Amir Weiss, MD

Laboratory for Research in Reproductive Sciences, Deprtment of Obstetrics and Gynecology, Ha'Emek Medical Center, Afula; Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel

Eliezer Shalev, MD

Laboratory for Research in Reproductive Sciences, Deprtment of Obstetrics and Gynecology, Ha'Emek Medical Center, Afula; Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel shaleve{at}tx.technion.ac.il

Objective: Pathologic placentation has been implicated in the pathogenesis of preeclamsia. We sought to assess the effect serum obtained from women with preeclampsia would have on JAR human choriocarcinoma cells regarding growth, invasiveness, and matrix metalloproteinase (MMP) secretion as compared to normotensive pregnant woman.

Methods: Blood was collected from 11 healthy pregnant women and from 10 patients with preeclampsia at 28-33 weeks of gestation. The JAR human choriocarcinoma cell line was cultured in the presence of 10% serum obtained from each group. Cell proliferation, invasiveness, and MMP secretion was measured using a cell proliferation kit, and Matrigel (BD Biosciences, Beit-Ha'Emek, Israel) invasion assay, and gel zymography, respectively.

Results: Cell growth increased by 6% when exposed to serum from patients with preeclampsia compared to 30% from controls (P < .01). Trophoblast invasion was significantly (P < .01) reduced in the preeclampsia group (21 ± 1.9%) compared to controls (27 ± 2.5%). Valid MMP-2 secretion was reduced by 51% in the preeclampsia group comapred to controls (P < .05).

Conclusion: Serum obtained from women with preeclampsia contains a factor or factors that exhibit an inhibitory effect on JAR trophoblast cell proliferation, invasiveness, and MMP-2 secretion. These factors may be involved in the pathologic placentation associated with the pathogenesis of preeclampsia.

Key Words: Preeclampsia • matrix metalloproteinase-2 • invasion assay • trophoblast

Journal of the Society for Gynecologic Investigation, Vol. 12, No. 6, e45-e50 (2005)
DOI: 10.1016/j.jsgi.2005.06.002
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