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Journal of the Society for Gynecologic Investigation, Vol. 12, No. 8, 558-562 (2005)
DOI: 10.1016/j.jsgi.2005.08.008

Amniotic Fluid Volume and Composition in Mouse Pregnancy

Cecilia Y. Cheung, PhD

Division of Perinatal Medicine, Department of Reproductive Medicine, University of California San Diego, La Jolla, California, USA; cycheung{at}ucsd.edu

Robert A. Brace, PhD

Division of Perinatal Medicine, Department of Reproductive Medicine, University of California San Diego, La Jolla, California, USA

Objective: The current study was undertaken to determine simultaneous changes in amniotic fluid (AF) volume and composition across gestation in the pregnant mouse.

Methods: Young adult mice (6 to 7 weeks old) of the CB6F1 strain were mated overnight. AF was collected on consecutive days from embryonic days 9.5 through 18.5 for measurements of volume and composition. Statistical analysis included one-factor analysis of variance (ANOVA).

Results: Af volume increased from 18 ± 4 (Se) µL on day 9.5 to a maximum of 147 ± 4 µL on days 15.5 to 16.5 and decreased sharply to 17 ± 3 µL on day 18.5 AF osmolality was unchanged except for a rise prior to delivery on day 19.5 to 20.5 AF sodium, calcium, and glucose concentrations increased and subsequently decreased as gestation progressed. AF potassium, chloride, and loctate concentrations initially decreased and then increased across gestation. Prior to day 9.5 and after day 18.5, AF volume was too small for volume or compositional determinations.

Conclusions: In the mouse, the rise in AF volume from mid gestation to a maximum late in gestation is similar to that in humans while the sharp fall prior to delivery is not. As observed in the fetal sheep, the changes in fluid volume are associated with aF osmolality and solute concentration changes that are correlated with advancing gestational age. These observations together with the feasibliity of quantifying AF volume and composition in the mouse fetus demonstate the possibility of using genetically altered mice as a model for future studies on the molecular mechanisms underlying the regulation of AE volume and composition.

Key Words: Mouse • amniotic fluid volume • osmolality • sodium • potassium • chloride • calcium • lactate • glucose


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