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Journal of the Society for Gynecologic Investigation, Vol. 12, No. 8, 563-569 (2005)
DOI: 10.1016/j.jsgi.2005.08.007

Ontogeny and Effects of Thyroid Hormone on ß1-Adrenergic Receptor mRNA Expression in Ovine Fetal Kidney Cortex

Jingfang Liu, PhD

Kai Chen, MD

Nancy K. Valego, PhD

Luke C. Carey, PhD

Departments of Obstetrics and Gynecology and Physiology and Pharmacology, Wake Forest University School of Medicine, Winston-Salem, North Carolina

James C. Rose, PhD

Departments of Obstetrics and Gynecology and Physiology and Pharmacology, Wake Forest University School of Medicine, Winston-Salem, North Carolina; jimrose{at}wfubmc.edu

Objective: Previous studies indicate that thyroidectomy (TX) decreases renin gene expression in ovine fetal renal cortex in late gestation. Fetal ovine renin-containing renocortical cells become increasingly responsive to ß-adrenergic stimulation as gestation proceeds. Increases in plasma thyroid hormone concentrations parallel this change, suggesting that there is a positive developmental relationship between the two. To examine this hypothesis, we determined the ontogeny of ß1-adrenergic receptor (ß1R) mRNA expression, and the effect of thyroid hormone on in vivo and in vitro expression in fetal sheep.

Methods: Renocortical tissue was obtained from naive, TX, and sham-operated fetuses to determine ß1R mRNA levels. Renin-containing renocortical cells from TX or sham fetuses were treated with isoproterenol (Iso) or forskolin (FSK) fory analysis of cellular cyclic adenosine monophophate (cAMP) levels. Renocortical cells from naive fetuses were treated with triiodothyronin (T3) to assess cellular ß1R mRNA levels. Fetal plasma thyroxine (T4) level was determined.

Results: Renocortical ß1R mRNA expression increased significantly between 100 and 140 days' gestational age (dGA), while TX attenuated this increase (p <.01). Renocortical cellular cAMP levels were higher in sham compared to TX fetuses following incubation with Iso or FSK (P <.05). Cells incubated with T3 exhibited significantly increased ß1R mRNA expression (P <.05).

Conclusion: The data suggest that thyroid hormone may be involved in modulating ovine fetal renocortical ß1R gene expression during development. We speculate that the increased ß1R mRNA expression in renal cortical cells as development progresses may mediate the increases in renin gene response to ß-adrenergic stimulation in late gestation.

Key Words: Thyroid hormone • ß1R mRNA • kidney • fetal development


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