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Placental Lactate Transporter Activity and Expression in Intrauterine Growth Restriction

Colin P. Sibley, PhD

Ian M. Doughty, PhD, FRCPCH

Tracey Johnston, MD, MRCOG

Jocelyn D. Glazier, PhD

Theresa L. Powell, PhD

Thomas Jansson, MD, PhD

Division of Human Development, University of Manchester, St. Mary's Hospital, Manchester, United Kingdom; Perinatal Center, Department of Physiology and Pharmacology, Gothenburg University, Gothenburg, Sweden; Department of Obstetrics and Gynecology, University of Cincinnati, Cincinnati, Ohio

Stephen W. D'Souza, MBChB, PhD, FRCP, FRCPCH

Division of Human Development, University of Manchester, St. Mary's Hospital, Manchester, United Kingdom; Perinatal Center, Department of Physiology and Pharmacology, Gothenburg University, Gothenburg, Sweden; Department of Obstetrics and Gynecology, University of Cincinnati, Cincinnati, Ohio; Division of Human Development, Academic Unit of Child Health, University of Manchester, St. Mary's Hospital, Manchester M13 0JH, United Kingdom sdesouza{at}man.ac.uk

Objectives: To compare lactate uptake in the microvillous plasma membrane (maternal facing [MVM]) in term and preterm placentas in intrauterine growth restriction (IUGR) and appropriate weight for gestational age (AGA) controls, and in the basal plasama membrane (fetal facing [BM]) at term. In addition, we examine the expression of monocarboxylate transporters (MCT1 and MCT4).

Methods: We measured [¹⁴C] L-lactate uptakes into vesides prepared from MVM and BM, stimulated by an inwardly directed H⁺ gradient. MCT expression was examined by western blotting.

Results: In term placentas, mean (± SE) [¹⁴C] L-lactate uptake into MVM vesicles of the IUGR (n = 6) and AGA (n = 11) groups at initial rate was similar (15.4 ± 2.3 versus 15.0 ± 1.1 pmol/mg protein/20 s). In preterm placentas, in IUGR (n = 3) and AGA (n = 3) groups, [¹⁴C] L-lactate uptake into MVM was also not significantly different. In BM vesicles from term placentas, [¹⁴C] L-lactate uptake was significantly lower in IUGR (n = 5) than in AGA (n = 6) controls (3.6 ± 0.4 versus 5.6 ± 0.6 pmol/mg protein/20 s, P <.05). MCT1 and MCT4 were expressed in BM vesicles, but there was no difference in expression betweern the IUGR and AGA groups.

Conclusions: These findings sugbgest that in IUGR placental lactate transport capacity in the BM is reduced, which may adversely affect placental lactate clearance.

Key Words: Human placenta • monocarboxylate • monocarboxylate transporter • SLC16 • intrauterine growth restriction

Journal of the Society for Gynecologic Investigation, Vol. 13, No. 5, 357-363 (2006)
DOI: 10.1016/j.jsgi.2006.04.006


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