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Insulin-like Growth Factor-I Regulates Vascular Endothelial Growth Factor Secretion by Human Oviductal Epithelial Cells and Stromal Fibroblasts

Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, Idaigaoka 1-1, Hasama-machi, Yufu-shi, Oita 879-5593, Japan nasu{at}med.oita-u.ac.jp

Hiroko Itoh, MD, PhD

Akitoshi Yuge, MD

Yasushi Kawano, MD, PhD

Hisashi Narahara, MD, PhD

Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, Oita, Japan

Objective: The aim of the current study is to evaluate the effect of insulin-like growth factor-I (IGF-I) on the production of vascular endothelial growth factor (VEGF) in the human fallopian tube.

Methods: Human oviductal epithelial cells (OEC) and oviductal stromal fibroblasts (OSF) were isolated from the ampullary sgement of the fallopian tubes of six premenopausal patients in the proliferative phase of the menstrual cycle. The secretion of VEGF₁₆₅ by cultured OEC and OSF in response to IGF-I was measured using an enzyme-linked immunosorbent assay (ELISA).

Results: The secretion of VEGF₁₆₅ was detected in cultured OEC and OSF under untreated conditions. The secretion of VEGF₁₆₅ was significantly stimulated with IGF-I administration in these cells.

Conclusion: The present findings suggest that IGF-I in the local environment may stimulate oviductal vascular permeability by inducing the production of VEGF by oviductal cells through autocrine and paracrine mechanisms. The modulation of the VEGF production in the fallopian tube may contribute to the normal and pathologic processes of oviductal fluid secretion by regulating oviductal vascular permeability during the menstrual cycle and in the peri-implantation period.

Key Words: Fallopian tube • insulin-like growth factor-I • vascular endothelial growth factor • oviductal fluid

Journal of the Society for Gynecologic Investigation, Vol. 13, No. 5, 368-371 (2006)
DOI: 10.1016/j.jsgi.2006.03.007


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