Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Journal of the Society for Gynecologic Investigation
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Zhang, X.
Right arrow Articles by Guo, Y.-L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, X.
Right arrow Articles by Li, X.-H.
Right arrow Articles by Ma, X.
Right arrow Articles by Wang, Z.-H.
Right arrow Articles by Lu, S.
Right arrow Articles by Guo, Y.-L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Redox-Induced Apoptosis of Human Oocytes in Resting Follicles In Vitro

Xiao Zhang, PhD

Xiao-Hong Li, PhD

Peking University First Hospital, Institute of Urology of Peking University; Reproductive and Genetic Center, First Hospital of Peking University; National Research Institute for Family Planning; Peking Union Medical College; and the World Health Organization Collaborative Center for Research in Human Reproduction, Beijing, P.R. China

Xu Ma, PhD

Peking University First Hospital, Institute of Urology of Peking University; Reproductive and Genetic Center, First Hospital of Peking University; National Research Institute for Family Planning; Peking Union Medical College; and the World Health Organization Collaborative Center for Research in Human Reproduction, Beijing, P.R. China; genetic{at}263.net.cn

Zhao-Huei Wang, PhD

Shuang Lu, PhD

Ying-Lu Guo, MD

Peking University First Hospital, Institute of Urology of Peking University; Reproductive and Genetic Center, First Hospital of Peking University; National Research Institute for Family Planning; Peking Union Medical College; and the World Health Organization Collaborative Center for Research in Human Reproduction, Beijing, P.R. China

Background: The age-related depletion of the resting follicle (RF) stock occurs as a result of two processes: atresia and entry in growth phase. Due to difficulties in obtaining sufficient numbers of RFs for study, little is known about the apoptotic mechanisms for RF atresia. The present study was designed to investigate the effects of oxidative stress on the apoptosis of RF oocytes.

Methods: RFs isolated from human adult ovaries were cultured in vitro, treated with H2O2 at various concentrations (50 µM, 100 µM, 1.0 mM) for 1 hour, and observed for up to 48 hours. The oxidant-induced apoptosis of oocytes were observed by detection of DNA fragments, mitochondria membrane potantial (MMP) and cytochrome c release.

Results: Based on nuclear morphology and TUNEL (terminal deoxynucleotidyl transferase-mediated dDTP nick end-labeling), oocyte apoptosis was observed in the RFs treated with 50 µM H2O2 with rates of 35% and 43% at 24 and 48 h after treatment, respectively. But intensive oxidative stress (1 mM H2O2) caused mainly necrosis as measured by quantifying propidium iodide (PI)-positive oocytes (44% within 12 hours), with lower level of apoptosis (17%) being observed at 24 hours after treatment. RFs treated with 100 µM H2O2 showed both apoptosis with the similar rate observed at 50µM and necrosis (13% PI-positive oocytes). Although pre-incubation with cyclosporine A (CsA) could effectively prevent oxidant-induced MMP collapse, but failed to suppress apoptosis of oocytes in RFs.

Conclusions: Oocytes in RFs in adult ovaries retain their ability to undergo apoptosis under oxidative stress, which is both dose- and time-dependent.

Key Words: Ovary • oocyte • oxidative stress • apoptosis • resting follicle

Journal of the Society for Gynecologic Investigation, Vol. 13, No. 6, 451-458 (2006)
DOI: 10.1016/j.jsgi.2006.05.005
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?