This Article Full Text (PDF) A correction has been published References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jackson, K. S. Articles by Fazleabas, A. T. Search for Related Content PubMed PubMed Citation Social Bookmarking                   What's this?

The Altered Distribution of the Steroid Hormone Receptors and the Chaperone Immunophilin FKBP52 in a Baboon Model of Endometriosis Is Associated With Progesterone Resistance During the Window of Uterine Receptivity

Department of Obstetrics and Gynecology (MC808), College of Medicine, University of Illinois at Chicago

Allison Brudney

Department of Obstetrics and Gynecology (MC808), College of Medicine, University of Illinois at Chicago

Julie M. Hastings

Department of Obstetrics and Gynecology (MC808), College of Medicine, University of Illinois at Chicago

Patricia A. Mavrogianis

Department of Obstetrics and Gynecology (MC808), College of Medicine, University of Illinois at Chicago

J. Julie Kim

Department of Obstetrics and Gynecology, Division of Reproductive Biology, Northwestern University, Chicago, Illinois

Asgerally T. Fazleabas

Department of Obstetrics and Gynecology (MC808), College of Medicine, University of Illinois at Chicago, asgi{at}uic.edu

This study examines the distribution of estrogen receptors (ESR), progesterone receptors (Pgr), and the chaperone immunophilin FKBP52 in the eutopic endometrium in a baboon model of endometriosis during the window of receptivity to determine if their aberrant distribution contributes to reduced fecundity. Endometriosis was induced by inoculation of menstrual endometrium into the peritoneal cavity. Eutopic endometrium was collected at 3, 6, 9, 12, and 15 months postinoculation. Western blot (WB) and immunohistochemical analyses were performed. Isolated endometrial stromal cells were cultured in the presence or absence of steroid hormones. In animals with endometriosis, ESR-1 (ER-) decreased in endometrial stromal cells, while ESR-2 (ER-ß) was reduced in both glandular epithelial (GE) and stromal cells. Immunoreactive total Pgr was markedly diminished in the GE, which was confirmed by WB analysis. Furthermore, treatment of isolated stromal cells from baboons with endometriosis with hormones did not increase levels of PRA or PRB as in control baboons. FKBP52 was also reduced in the eutopic endometrium of baboons with endometriosis. Endometriosis results in an aberrant distribution of ESR-1, ESR-2, Pgr, and FKBP52 in the eutopic endometrium. The authors propose that a dysregulation in the paracrine signaling between the endometrial stromal and GE cells reduces the responsiveness of Pgr, creating an endometrial environment that is unsuitable for implantation.

Key Words: KEY WORDS: Endometriosis • steroid receptors,immunohistochemistry • baboon • endometrial receptivity • uterus.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				D.K. Hapangama, M.A. Turner, J.A. Drury, S. Quenby, G. Saretzki, C. Martin-Ruiz, and T. Von Zglinicki Endometriosis is associated with aberrant endometrial expression of telomerase and increased telomere length Hum. Reprod., July 1, 2008; 23(7): 1511 - 1519. [Abstract] [Full Text] [PDF]

				O. Guzeloglu-Kayisli, G. Halis, S. Taskiran, U. A. Kayisli, and A. Arici DNA-Binding Ability of NF-{kappa}B is Affected Differently by ER{alpha} and ER{beta} and Its Activation Results in Inhibition of Estrogen Responsiveness Reproductive Sciences, May 1, 2008; 15(5): 493 - 505. [Abstract] [PDF]

				F. Minici, F. Tiberi, A. Tropea, M. Orlando, M. F. Gangale, F. Romani, S. Campo, A. Bompiani, A. Lanzone, and R. Apa Endometriosis and human infertility: a new investigation into the role of eutopic endometrium Hum. Reprod., March 1, 2008; 23(3): 530 - 537. [Abstract] [Full Text] [PDF]