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Human Nonmetastatic Clone 23 Type 1 Gene Suppresses Migration of Cervical Cancer Cells and Enhances the Migration Inhibition of Fungal Immunomodulatory Protein From Ganoderma tsugae

Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung, Taiwan, School of Medicine, Chung Shan Medical University, Taichung, Taiwan, Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan

Shun-Fa Yang, PhD

Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan

Gin-Den Chen, MD

Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung, Taiwan, School of Medicine, Chung Shan Medical University, Taichung, Taiwan

Chih-Ping Han, MD, PhD

Clinical Research Center, Chung Shan Medical University Hospital, Taichung, Taiwan, Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan, Department of Pathology, Chung Shan Medical University, Taichung, Taiwan

Shiuan-Chih Chen, MD, PhD

Department of Family Medicine Chung Shan Medical University Hospital, Taichung, Taiwan

Long-Yau Lin, MD, PhD

Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung, Taiwan, School of Medicine, Chung Shan Medical University, Taichung, Taiwan, Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan

Jiunn-Liang Ko, PhD

Institute of Medical and Molecular Toxicology Chung Shan Medical University, Taichung, Taiwan, ming84921345{at}yahoo.com.tw

The authors investigate the effects of human nonmetastatic clone 23 type 1 (nm23-H1 ) gene and fungal immunomodulatory protein—Ganoderma tsugae (FIP-gts) on the metastatic potential of cervical cancer cells and assess whether nm23-H1 can influence the action of FIP-gts using cell migration and invasion assays and gelatin zymography. The nm23-H1 gene was stably transfected into Caski cells, which lacked nm23-H1 expression. The results show that nm23-H1 stably transfected Caski cells exhibit reduced cell migration but no change of cell invasion and matrix metalloproteinase (MMP)—2 and —9 activities. FIP-gts reduced cell migration in SiHa and nm23-H1 transfected Caski cells more significantly compared with Caski cells and reduced invasion in Caski and nm23-H1—transfected Caski cells, but it exerted no influence on MMP-2 and MMP-9 activities in them. Conclusively, the nm23-H1 gene suppresses cervical cancer cell migration but not invasion and activities of MMP-2 and MMP-9 and enhances the inhibition of FIP-gts upon migration.

Key Words: Human nonmetastatic clone 23 type 1 gene • fungal immunomodulatory protein Ganoderma tsugae • cervical cancer cells • cell migration • cell invasion • matrix metalloproteinase.

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