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Reproductive Sciences
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Modulation of Monocyte Chemotactic Protein-1 Expression During Lipopolysaccharide-Induced Preterm Delivery in the Pregnant Mouse

Allaire K. Diamond, MEd

Department of Obstetrics and Gynecology, University of Vermont College of Medicine, Burlington

Leigh M. Sweet, BA

Department of Obstetrics and Gynecology, University of Vermont College of Medicine, Burlington

Karen H. Oppenheimer, BA

Department of Obstetrics and Gynecology, University of Vermont College of Medicine, Burlington

Diana F. Bradley, BS

Huntsman Cancer Institute, University of Utah, Salt Lake City

Mark Phillippe, MD, MHCM

Department of Obstetrics and Gynecology, University of Vermont College of Medicine, Burlington, mark.phillippe{at}uvm.edu

Preterm delivery is often associated with increased cytokine and chemokine production. These studies characterize the expression of the chemokine monocyte chemotactic protein-1 (MCP-1) in mice during lipopolysaccharide (LPS)—induced preterm delivery. Uterine and other tissues were harvested from CD-1 mice on gestational day 15 after intrauterine LPS injection. Quantitative real-time reverse-transcriptase polymerase chain reactions determined MCP-1 and toll-like receptor 4 (TLR4) mRNA expression during the 24 hours after LPS. MCP-1 protein expression was determined using a cytokine/chemokine protein array, enzyme-linked immunosorbant assay, and immunohistochemistry. Intrauterine LPS injection caused preterm delivery in CD-1 mice between 12 and 24 hours. Expression of MCP-1 mRNA significantly increased at 2 and 6 hours, while TLR4 expression did not significantly change over 24 hours. The MCP-1 protein levels peaked by 2 to 6 hours in maternal serum, liver, lung, kidney, and uterus. Immunohistochemistry confirmed MCP-1 in the myometrium and endometrium. These studies provide evidence suggesting that MCP-1 potentially plays an important role during the proinflammatory immune response, leading to preterm labor in the mouse.

Key Words: CD-1 mouse • preterm delivery • quantitative reverse-transcriptase polymerase chain reaction • chemokines.

Reproductive Sciences, Vol. 14, No. 6, 548-559 (2007)
DOI: 10.1177/1933719107307792
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