Reproductive Sciences

 

Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

http://mc.manuscriptcentral.com/rsci

SAGETRACK

Sign In to gain access to subscriptions and/or personal tools.
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (1)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Diamond, A. K.
Right arrow Articles by Phillippe, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Diamond, A. K.
Right arrow Articles by Sweet, L. M.
Right arrow Articles by Oppenheimer, K. H.
Right arrow Articles by Bradley, D. F.
Right arrow Articles by Phillippe, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Reproductive Sciences, Vol. 14, No. 6, 548-559 (2007)
DOI: 10.1177/1933719107307792

Modulation of Monocyte Chemotactic Protein-1 Expression During Lipopolysaccharide-Induced Preterm Delivery in the Pregnant Mouse

Allaire K. Diamond, MEd

Department of Obstetrics and Gynecology, University of Vermont College of Medicine, Burlington

Leigh M. Sweet, BA

Department of Obstetrics and Gynecology, University of Vermont College of Medicine, Burlington

Karen H. Oppenheimer, BA

Department of Obstetrics and Gynecology, University of Vermont College of Medicine, Burlington

Diana F. Bradley, BS

Huntsman Cancer Institute, University of Utah, Salt Lake City

Mark Phillippe, MD, MHCM

Department of Obstetrics and Gynecology, University of Vermont College of Medicine, Burlington, mark.phillippe{at}uvm.edu

Preterm delivery is often associated with increased cytokine and chemokine production. These studies characterize the expression of the chemokine monocyte chemotactic protein-1 (MCP-1) in mice during lipopolysaccharide (LPS)—induced preterm delivery. Uterine and other tissues were harvested from CD-1 mice on gestational day 15 after intrauterine LPS injection. Quantitative real-time reverse-transcriptase polymerase chain reactions determined MCP-1 and toll-like receptor 4 (TLR4) mRNA expression during the 24 hours after LPS. MCP-1 protein expression was determined using a cytokine/chemokine protein array, enzyme-linked immunosorbant assay, and immunohistochemistry. Intrauterine LPS injection caused preterm delivery in CD-1 mice between 12 and 24 hours. Expression of MCP-1 mRNA significantly increased at 2 and 6 hours, while TLR4 expression did not significantly change over 24 hours. The MCP-1 protein levels peaked by 2 to 6 hours in maternal serum, liver, lung, kidney, and uterus. Immunohistochemistry confirmed MCP-1 in the myometrium and endometrium. These studies provide evidence suggesting that MCP-1 potentially plays an important role during the proinflammatory immune response, leading to preterm labor in the mouse.

Key Words: CD-1 mouse • preterm delivery • quantitative reverse-transcriptase polymerase chain reaction • chemokines.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
V. M. Abrahams, P. B. Aldo, S. P. Murphy, I. Visintin, K. Koga, G. Wilson, R. Romero, S. Sharma, and G. Mor
TLR6 Modulates First Trimester Trophoblast Responses to Peptidoglycan
J. Immunol., May 1, 2008; 180(9): 6035 - 6043.
[Abstract] [Full Text] [PDF]