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Evaluation of Matrix Metalloproteinase—2 Expression in Cervical Carcinogenesis Using Tissue Array and Integrated Optical Density for Immunoreactivity

Department of Obstetrics and Gynecology Chung Shan Medical University, Taichung Taiwan, Institute of Medicine Chung Shan Medical University, Taichung Taiwan

Chih-Ping Han, MD, PhD

Institute of Medicine Chung Shan Medical University, Taichung Taiwan, Clinical Research Center Chung Shan Medical University, Taichung Taiwan, Department of Pathology Chung Shan Medical University, Taichung Taiwan

Jiunn-Liang Ko, PhD

Institute of Medical and Molecular Toxicology Chung Shan Medical University, Taichung, Taiwan

Gin-Den Chen, PhD

Department of Obstetrics and Gynecology Chung Shan Medical University, Taichung Taiwan, School of Medicine Chung Shan Medical University, Taichung Taiwan

Shun-Fa Yang, PhD

Institute of Medicine Chung Shan Medical University, Taichung Taiwan

Shiuan-Chih Chen, MD, PhD

Department of Family Medicine Chung Shan Medical University, Taichung, Taiwan

Horng-Jyh Tsai, MD, PhD

Department of Obstetrics and Gynecology Chung Shan Medical University, Taichung Taiwan, School of Medicine Chung Shan Medical University, Taichung Taiwan, Institute of Medicine Chung Shan Medical University, Taichung Taiwan

Long-Yau Lin, MD, PhD

Department of Obstetrics and Gynecology Chung Shan Medical University, Taichung Taiwan, School of Medicine Chung Shan Medical University, Taichung Taiwan, Institute of Medicine Chung Shan Medical University, Taichung Taiwan

Po-Hui Wang, MD, PhD

Department of Obstetrics and Gynecology Chung Shan Medical University, Taichung Taiwan, School of Medicine Chung Shan Medical University, Taichung Taiwan, Institute of Medicine Chung Shan Medical University, Taichung Taiwan, lin12st653{at}yahoo.com.tw

To correlate matrix metalloproteinase—2 (MMP-2) expression with cervical carcinogenesis, the authors use reverse-transcription polymerase chain reaction to detect MMP-2 mRNA expression in 10 cervical squamous cell carcinoma (SCC), 10 high-grade cervical intraepithelial neoplasia (CIN), and 10 normal tissues. They further detect MMP-2 immunoreactivity of 24 tissue cores in each SCC, high-and low-grade CINs, and a healthy subgroup on a tissue array using integrated optical density (IOD) for number and intensity of stained cells in 345 x 345 pixels. They found the mRNA expression of MMP-2 to be higher in most SCCs (9/10 samples) and high-grade CINs (7/10 samples) but lower in normal tissues. The IOD of MMP-2 was significantly higher in high-grade CIN than in normal and low-grade CIN tissue cores (P < .001 for both) and significantly higher in SCC than in high-grade CIN tissue cores (P < .001). The results show that MMP-2 upregulation confers on tumor cells the ability to degrade the subepithelial basement membrane and subsequently invade the cervix.

Key Words: Matrix metalloproteinase—2,squamous cell carcinoma • high-grade cervical intraepithelial neoplasia • tissue array • integrated optical density.

Reproductive Sciences, Vol. 14, No. 7, 719-726 (2007)
DOI: 10.1177/1933719107307537


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