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This version was published on February 1, 2008
Reproductive Sciences, Vol. 15, No. 2, 189-194 (2008)
DOI: 10.1177/1933719107310710

Interleukin-1β Secretion From Cord Blood Mononuclear Cells In Vitro Involves P2X7 Receptor Activation

Averil Y. Warren, MPhil

Centre for Reproduction and Early Life, Institute for Clinical Research, University of Nottingham, UK

Laura Harvey, BMBS

Centre for Reproduction and Early Life, Institute for Clinical Research, University of Nottingham, UK

Robert W. Shaw, MD

Centre for Reproduction and Early Life, Institute for Clinical Research, University of Nottingham, UK

Raheela N. Khan, PhD

Centre for Reproduction and Early Life, Institute for Clinical Research, University of Nottingham, UK, raheela.khan{at}nottingham.ac.uk

The purinergic receptor P2X7 is activated by extracellular adenosine 5'-triphosphate (ATP) and promotes the efficient release of interleukin-1β. The authors examine protein and molecular expression of the P2X7 receptor and its ability to stimulate interleukin-1β release in cord blood mononuclear cells (CBMCs) from placentae of term nonlaboring and laboring women. They show both mRNA and protein (78 kDa) expression for the P2X 7 receptor in CBMCs of parturient and nonparturient women. Costimulation of CBMCs in vitro with bacterial endotoxin and ATP resulted in significantly (P < .05) enhanced interleukin-1β release in laboring (54.17 ± 24.78 pg/mL—1; n = 8) compared with nonlaboring (13.60 ± 3.20 pg/mL—1; n = 7) deliveries. Release of interleukin-1β in both groups was blocked by preincubation with oxidized ATP, a P2X7 receptor antagonist. The authors provide evidence for a novel inflammatory pathway in the release of interleukin-1β, which may be linked to the onset of labor.

Key Words: Labor • cytokine • interleukin-1β • cord blood • P2X7 receptor.


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