Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to register

SAGETRACK

Sign In to gain access to subscriptions and/or personal tools.
Reproductive Sciences
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
1933719107310398v1
15/2/210    most recent
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via ISI Web of Science (1)
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Salih, S. M.
Right arrow Articles by Al-Hendy, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Salih, S. M.
Right arrow Articles by Salama, S. A.
Right arrow Articles by Jamaluddin, M.
Right arrow Articles by Fadl, A. A.
Right arrow Articles by Blok, L. J.
Right arrow Articles by Burger, C. W.
Right arrow Articles by Nagamani, M.
Right arrow Articles by Al-Hendy, A.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Hazardous Substances DB
*PROGESTERONE
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Progesterone-Mediated Regulation of Catechol-O-Methyl Transferase Expression in Endometrial Cancer Cells

Sana M. Salih, MD

Department of Obstetrics & Gynecology, University of Wisconsin, Madison

Salama A. Salama, PhD

Departments of Obstetrics & Gynecology, University of Texas Medical Branch, sasalama{at}utmb.edu

Mohammad Jamaluddin, PhD

Department of Internal Medicine University of Texas Medical Branch, Galveston

Amin A. Fadl, PhD

Department of Pathobiology, Tuskegee University College of Veterinary Medicine, Tuskegee, Alabama

Leen J. Blok, PhD

Department of Obstetrics and Gynecology, Erasmus Medical Center, Rotterdam,The Netherlands

Curt W. Burger, PhD

Department of Obstetrics and Gynecology, Erasmus Medical Center, Rotterdam,The Netherlands

Manubai Nagamani, MD

Departments of Obstetrics & Gynecology, University of Texas Medical Branch

Ayman Al-Hendy, PhD, MD

Departments of Obstetrics & Gynecology, University of Texas Medical Branch

The effects of estrogen and progesterone on the expression of estrogen-metabolizing enzymes such as catechol-O-methyl transferase (COMT) are not known. COMT converts genotoxic catecholestrogens to anticarcinogenic methoxyestrogens in the endometrium. The aim of this study is to investigate the effect of progesterone on COMT expression in well-differentiated endometrial cancer cells. The wild-type Ishikawa cell line as well as progesterone receptor A— or progesterone receptor B—transfected Ishikawa cells were used for in vitro studies. The regulation of COMT expression by progesterone was studied using Western blots, Hoechst dye DNA proliferation studies, and wild-type and/or site-directed mutagenesis of COMT promoter 1—luciferase reporter gene. Progesterone upregulated COMT protein expression in Ishikawa cells through progesterone receptor A isoform. COMT promoter activity was differentially regulated by the 3 half-site progesterone response elements in the COMT promoter. High doses of 2-ME2 inhibited Ishikawa cell proliferation. These data suggest that COMT expression is hormonally regulated in well-differentiated human endometrial cancer cells. COMT regulation and 2-ME2 production in the endometrium may affect endometrial carcinogenesis.

Key Words: Catechol-o-methyl transferase • progesterone • endometrial cancer.

This version was published on February 1, 2008

Reproductive Sciences, Vol. 15, No. 2, 210-220 (2008)
DOI: 10.1177/1933719107310398
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?