Reproductive Sciences

 

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This version was published on April 1, 2008
Reproductive Sciences, Vol. 15, No. 4, 366-373 (2008)
DOI: 10.1177/1933719107312627

Altered Endothelin Receptor Binding in Response to Nitric Oxide Synthase Inhibition in the Pregnant Rat

Mark G. Neerhof, DO

Departments of Obstetrics and Gynecology, Evanston Northwestern Healthcare

Tamas Jilling, PhD

Department of Pediatrics Evanston Northwestern Healthcare, Evanston, Illinois, Northwestern University Feinberg School of Medicine, Chicago, Illinois

Sylvia Synowiec, BS

Departments of Obstetrics and Gynecology, Evanston Northwestern Healthcare

Saira Khan, MS

Departments of Obstetrics and Gynecology, Evanston Northwestern Healthcare

Larry G. Thaete, PhD

Departments of Obstetrics and Gynecology, Evanston Northwestern Healthcare, lthaete{at}enh.org

The authors evaluate the expression of endothelin-1 (ET-1) and its receptors in the uterus and placenta during maternal nitric oxide synthase (NOS) inhibition. Timed-pregnant rats received L-NAME (2.5 mg/kg/h) or saline from day 14 to 21 of gestation. Uterine and placental tissues collected on day 21 were assayed for preproET-1, ET A, and ETB mRNA expression; localization and expression of ET-1 and receptor proteins; and receptor activity. NOS inhibition did not affect preproET-1 mRNA expression in the placenta or uterus. ETA expression decreased in the uterine free wall, but no other changes in receptor mRNA expression were observed in the uterus or placenta. ET-1 and receptor proteins were unchanged. Placental ETA and ETB receptor binding decreased. Uterine ETA receptor binding decreased in the placental bed. ET-1, a prominent mediator during NOS inhibition, is not of uterine or placental origin. Reduced receptor binding activity is the primary means by which these tissues regulate their response to ET-1 in the setting of NOS inhibition.

Key Words: Endothelin • nitric oxide • placenta • pregnancy • uterus • rat.


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