Reproductive Sciences

 

Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

http://mc.manuscriptcentral.com/rsci

SAGETRACK

Sign In to gain access to subscriptions and/or personal tools.
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Google Scholar
Right arrow Articles by McIntire, R. H.
Right arrow Articles by Hunt, J. S.
PubMed
Right arrow PubMed Citation
Right arrow Articles by McIntire, R. H.
Right arrow Articles by Ganacias, K. G.
Right arrow Articles by Hunt, J. S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Reproductive Sciences, Vol. 15, No. 5, 437-447 (2008)
DOI: 10.1177/1933719107314065

Programming of Human Monocytes by the Uteroplacental Environment

Ramsey H. McIntire, PhD

Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas, mcintirer{at}obgyn.ucsf.edu

Karen G. Ganacias, BS

Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas

Joan S. Hunt, PhD, DSc

Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas

During human pregnancy, monocytes recruited to the uterus (decidua) are modified to promote immune defense and semiallogeneic pregnancy. The purpose of this study was to identify decidual factors involved in programming of monocytes into decidual macrophages by comparing the surface and secretory phenotypes of resting and interferon- {gamma} (IFN-{gamma})—activated monocytes, unfractionated decidual cells, purified term decidual macrophages, and monocyte-derived macrophages. Surface markers for antigen presentation (HLA-DR, CD86), a membrane-bound cytokine interleukin (IL)—15, leukocyte immunoglobulin-like receptors (LILRB1, LILRB2), and secreted anti-inflammatory cytokines (transforming growth factor [TGF]—β1 and IL-10) were assessed. The results demonstrate that differentiated, activated monocytes closely resemble but are not identical to decidual macrophages. In addition to differential IFN-{gamma} responsiveness, decidual macrophages were smaller than monocyte-derived macrophages and produced IL-10, which monocyte-derived macrophages did not. Only the unfractionated decidual cells secreted TGF-β1. These results suggest that activation, differentiation, and decidual signals cooperate to program monocytes into the decidual macrophage phenotype.

Key Words: Activation • cell surface markers • cytokines • differentiation • leukocyte immunoglobulin-like receptors (LILR).


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?