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Reproductive Sciences
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Expression and Distribution of Glucocorticoid Receptors in the Ovine Fetal Adrenal Cortex: Effect of Long-term Hypoxia

Brandon Root, BA

University of Redlands, Redlands, California

Jenna Abrassart, BA

University of Redlands, Redlands, California

Dean A. Myers, PhD

Department of Obstetrics/Gynecology, University of Oklahoma Health Sciences Center, Oklahoma City

Tshepo Monau, MS

Center for Perinatal Biology, Loma Linda University, Loma Linda, California

Charles A. Ducsay, PhD

Center for Perinatal Biology, Loma Linda University, Loma Linda, California, cducsay{at}llu.edu

This study was designed to determine if long-term hypoxia (LTH) alters adrenal glucocorticoid receptor (GR) expression in the ovine fetal adrenal cortex. Ewes were maintained at 3820 m from ~30 to 138 to 140 days' gestation, and fetal adrenals were collected. Western analysis revealed two ~94-kDa GR-{alpha} isoforms and a lower molecular weight (45 kDa) form. A decreasing trend in the ratio of 94-kDa/45-kDa bands following LTH suggested an increase in GR turnover. Immunohistochemistry demonstrated dense GR staining in the zona glomerulosa with minimal staining in the zona fasciculata in the control group, while dense staining was observed throughout the cortex in LTH. Western analysis and reverse transcription polymerase chain reaction confirmed that the GR- β isoform is not present or expressed at extremely low levels in the fetal adrenal, hypothalamus, pituitary, and placenta. These data indicate that LTH alters GR-{alpha} function in the fetal adrenal cortex and suggest that GR-β is not expressed in sheep.

Key Words: Adrenal • fetus,sheep,glucocorticoid receptor • hypoxia.

Reproductive Sciences, Vol. 15, No. 5, 517-528 (2008)
DOI: 10.1177/1933719107311782
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T. R. Monau, V. E. Vargas, N. King, S. M. Yellon, D. A. Myers, and C. A. Ducsay
Long-Term Hypoxia Increases Endothelial Nitric Oxide Synthase Expression in the Ovine Fetal Adrenal
Reproductive Sciences, September 1, 2009; 16(9): 865 - 874.
[Abstract] [PDF]