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Gene Expression Profiling Reveals Putative HOXA10 Downstream Targets in the Periimplantation Mouse Uterus

Division of Reproductive Endocrinology and Infertility, Yale University School of Medicine, New Haven, Connecticut

Robert Pinard, PhD

Division of Reproductive Endocrinology and Infertility, Yale University School of Medicine, New Haven, Connecticut

Hugh S. Taylor, MD

Division of Reproductive Endocrinology and Infertility, Yale University School of Medicine, New Haven, Connecticut, hugh.taylor{at}yale.edu

HOXA10 encodes a transcription factor required for endometrial receptivity and embryo implantation. The objective of this study was to identify and to characterize those molecular markers regulated by HOXA10 expression. The authors have identified putative HOXA10 target genes identified by microarray analysis employing a murine model of transient HOXA10 expression during the anticipated implantation window. Microarray analysis identified 40 statistically significant genes regulated by HOXA10 overexpression of which 31 genes were downregulated greater than 2-fold over control and 9 genes were upregulated. Cellular ontogenies of differentially expressed genes include cell adhesion molecules, signal transduction factors, and metabolic regulators. Semiquantitative real-time reverse transcriptase polymerase chain reaction confirmed regulation of selected candidate genes. Examples included clusterin (Clu), phoshoglycerate 3-dehydrogenase (3-Pgdh), and tumor-associated calcium signal transducer 2 (Tacstd2). Elucidation of these pathways will allow further characterization of the molecular mechanisms governing endometrial development, which also may function to enhance uterine receptivity.

Key Words: Implantation • HOXA10,3-phosphoglycerate dehydrogenase • microarray • endometrium.

Reproductive Sciences, Vol. 15, No. 5, 529-535 (2008)
DOI: 10.1177/1933719108316911


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