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Reproductive Sciences
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The Role of PLCβ1 in the Control of Oocyte Meiosis During Folliculogenesis

Arlette Pesty, PhD

UMR-S 566, CEA, DSV/iRCM/SCSR/LGAG; INSERM, Unité 566; Université Denis Diderot-Paris 7; Université Paris-Sud F-92260, Fontenay aux Roses, France

Ophélie Broca

AP-HP, Groupe hospitalier Pitié Salpêtrière, UF Biologie de la Reproduction, Université Pierre et Marie Curie-Paris 6, Paris, F-75013 France

Catherine Poirot, PhD, MCU-PH

AP-HP, Groupe hospitalier Pitié Salpêtrière, UF Biologie de la Reproduction, Université Pierre et Marie Curie-Paris 6, Paris, F-75013 France

Brigitte Lefèvre, PhD, DR

UMR-S 566, CEA, DSV/iRCM/SCSR/LGAG; INSERM, Unité 566; Université Denis Diderot-Paris 7; Université Paris-Sud F-92260, Fontenay aux Roses, France, brigitte.lefevre{at}cea.fr, AP-HP, Groupe hospitalier Pitié Salpêtrière, UF Biologie de la Reproduction, Université Pierre et Marie Curie-Paris 6, Paris, F-75013 France

The dynamics of the subcellular distribution of PLCβ1 was investigated during meiosis competence acquisition and meiosis resumption in relation to oocyte diameter and to nonsurrounded-nucleolus or surrounded-nucleolus chromatin configurations. Oocytes collected after both in vivo and in vitro folliculogenesis were studied. In both conditions, at the beginning of the process, most oocytes exhibited a nuclear PLCβ 1 associated with a nonsurrounded-nucleolus chromatin configuration. Then at the final stage of the process, the factors shifted mainly toward a cytoplasmic PLCβ1 and a surrounded-nucleolus chromatin configuration, typical of a preovulatory fertilizable oocyte. Additionally, only germinal vesicle oocytes with a diameter > 75 µm, and exhibiting cytoplasmic PLC β1 distribution and surrounded-nucleolus chromatin configuration, resumed meiosis. Our findings demonstrate a strong correlation between oocyte diameter, chromatin configuration, and PLCβ1 localization. Thus, PLCβ1 localization appears to be a key factor determining the progressive acquisition of meiotic competence and final resumption of meiosis.

Key Words: Oocyte • PLCβ1 • meiosis • folliculogenesis • chromatin • growth • mouse.

Reproductive Sciences, Vol. 15, No. 7, 661-672 (2008)
DOI: 10.1177/1933719108322434
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