This Article Full Text (PDF) All Versions of this Article: 1933719108317585v1 15/8/797    most recent References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Savaris, R. F. Articles by Giudice, L. C. Search for Related Content PubMed PubMed Citation Articles by Savaris, R. F. Articles by Hamilton, A. E. Articles by Lessey, B. A. Articles by Giudice, L. C. Social Bookmarking                   What's this?

Endometrial Gene Expression in Early Pregnancy: Lessons From Human Ectopic Pregnancy

Departmento de Obstetrícia e Ginecologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

Amy E. Hamilton, BS

Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, California

Bruce A. Lessey, MD, PhD

Department of Obstetrics and Gynecology, University Medical Group, Greenville Hospital System, Greenville, South Carolina

Linda C. Giudice, MD, PhD, MSc

Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, California, giudice{at}obgyn.ucsf.edu

Human endometrium undergoes modifications in preparation for embryonic implantation. This study investigated in vivo the endocrine effects of pregnancy on the endometrium, using the model of ectopic pregnancy. Endometrial biopsies from 9 subjects with ectopic pregnancy (Preg) were compared with 8 and 6 samples of mid and late secretory endometrium, respectively. After hybridizing with Affymetrix HGU133 Plus 2 chips, data were analyzed using GeneSpring GX and Ingenuity Pathways Analysis. From 54 675genes, 3021 genes were significantly differentiated when mid-secretory endometrium was compared with the Preg (Volcano plot; P < .05, 2-fold change).The complement and coagulation cascade, phospholid degradation, glycosphingolipid biosynthesis (globoseries), retinol metabolism, antigen presentation pathway, glycosphingolipid biosynthesis, and O-glycan biosynthesis were main significant canonical pathways found in Preg samples. Validation was done with reverse transcriptase polymerase chain reaction. In conclusion, the ectopic embryo has a significant impact, by an endocrine mechanism, on endometrium, when compared with the window of implantation.

Key Words: Microarray • ectopi cpregnancy • transcriptome.

This version was published on October 1, 2008

Reproductive Sciences, Vol. 15, No. 8, 797-816 (2008)
DOI: 10.1177/1933719108317585


CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A.W. Horne, W.C. Duncan, A.E. King, S. Burgess, P.C. Lourenco, P. Cornes, P. Ghazal, A.R. Williams, L. Udby, and H.O.D. Critchley Endometrial cysteine-rich secretory protein 3 is inhibited by human chorionic gonadotrophin, and is increased in the decidua of tubal ectopic pregnancy Mol. Hum. Reprod., May 1, 2009; 15(5): 287 - 294. [Abstract] [Full Text] [PDF]

				I. Van Vaerenbergh, L. Van Lommel, V. Ghislain, P. In't Veld, F. Schuit, H. M. Fatemi, P. Devroey, and C. Bourgain In GnRH antagonist/rec-FSH stimulated cycles, advanced endometrial maturation on the day of oocyte retrieval correlates with altered gene expression Hum. Reprod., May 1, 2009; 24(5): 1085 - 1091. [Abstract] [Full Text] [PDF]