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Endometrial Gene Expression in Early Pregnancy: Lessons From Human Ectopic Pregnancy

Departmento de Obstetrícia e Ginecologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

Amy E. Hamilton, BS

Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, California

Bruce A. Lessey, MD, PhD

Department of Obstetrics and Gynecology, University Medical Group, Greenville Hospital System, Greenville, South Carolina

Linda C. Giudice, MD, PhD, MSc

Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, California, giudice{at}obgyn.ucsf.edu

Human endometrium undergoes modifications in preparation for embryonic implantation. This study investigated in vivo the endocrine effects of pregnancy on the endometrium, using the model of ectopic pregnancy. Endometrial biopsies from 9 subjects with ectopic pregnancy (Preg) were compared with 8 and 6 samples of mid and late secretory endometrium, respectively. After hybridizing with Affymetrix HGU133 Plus 2 chips, data were analyzed using GeneSpring GX and Ingenuity Pathways Analysis. From 54 675genes, 3021 genes were significantly differentiated when mid-secretory endometrium was compared with the Preg (Volcano plot; P < .05, 2-fold change).The complement and coagulation cascade, phospholid degradation, glycosphingolipid biosynthesis (globoseries), retinol metabolism, antigen presentation pathway, glycosphingolipid biosynthesis, and O-glycan biosynthesis were main significant canonical pathways found in Preg samples. Validation was done with reverse transcriptase polymerase chain reaction. In conclusion, the ectopic embryo has a significant impact, by an endocrine mechanism, on endometrium, when compared with the window of implantation.

Key Words: Microarray • ectopi cpregnancy • transcriptome.

This version was published on October 1, 2008

Reproductive Sciences, Vol. 15, No. 8, 797-816 (2008)
DOI: 10.1177/1933719108317585


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