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Reproductive Sciences
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Pathogenic Reactions of the Ovarian Surface Epithelium to Ovulation, Dimethylbenzanthracene, and Estrogen are Negated by Vitamin E

William J. Murdoch, PhD

Reproductive Biology Program, University of Wyoming, Laramie, Wyoming, wmurdoch{at}uwyo.edu

Edward A. Van Kirk, MS

Reproductive Biology Program, University of Wyoming, Laramie, Wyoming

Youqing Shen, PhD

Reproductive Biology Program, University of Wyoming, Laramie, Wyoming

Objective: Acute effects of ovulation, the tumor suppressor disruptor dimethylbenzanthracene, the mitogen estradiol-17β, and the antioxidant vitamin E on the ovarian epithelium were assessed in seasonally anestrous ewes. Methods: Dimethylbenzanthracene and vitamin E were administered before and estradiol after gonadotropin-induced ovulation. Ovaries were removed for histopathology one and three weeks following ovulation. Surface epithelial cells isolated at one week were immunostained for 8-oxoguanine and assayed for urokinase plasminogen activator secretion—markers of genotoxic and invasive potentials. Results: Ovarian surface invaginations and cortical inclusion cysts containing stratified epithelium were observed at three weeks in ovulated animals treated with dimethylbenzanthracene and estradiol. Progenitor cells were 8-oxoguanine positive and hypersecreted urokinase plasminogen activator. Untoward responses were circumvented by vitamin E. Conclusion: Antioxidant therapy appears to be of value in precluding periovulatory disturbances to the ovarian surface epithelium that has been associated with carcinogenesis.

Key Words: Ovarian surface epithelium • ovulation • carcinogen • steroid hormones • vitamin E • sheep.

Reproductive Sciences, Vol. 15, No. 8, 839-845 (2008)
DOI: 10.1177/1933719108322435
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