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Reproductive Sciences
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Autophagy-Related Proteins, LC3 and Beclin-1, in Placentas From Pregnancies Complicated by Preeclampsia

Soo-Young Oh, MD

Department of Obstetrics and Gynecology, Sungkyunkwan University School of Medicine, Seoul, South Korea, crroh{at}skku.edu

Suk-Joo Choi, MD

Department of Obstetrics and Gynecology, Sungkyunkwan University School of Medicine, Seoul, South Korea

Kyung Hee Kim

Department of Obstetrics and Gynecology, Sungkyunkwan University School of Medicine, Seoul, South Korea

EunYoon Cho, MD

Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea

Jong-Hwa Kim, MD

Department of Obstetrics and Gynecology, Sungkyunkwan University School of Medicine, Seoul, South Korea

Cheong-Rae Roh, MD

Department of Obstetrics and Gynecology, Sungkyunkwan University School of Medicine, Seoul, South Korea

The objective of this study is to investigate the expression of autophagy-related proteins (LC3 and beclin-1) in human placentas and the changes they undergo in the placentas from pregnancies complicated by preeclampsia (PE) and to explore the regulatory mechanisms of these proteins in JEG-3 cells in response to hypoxia or cytokine treatment.The presence of autophagosomes was confirmed with electron microscopy and the expression of LC3 and beclin-1 by immunoimaging methods in human placental trophoblasts. Compared with the placentas from normal pregnancies, the expression of LC3-II protein, but not beclin-1, was increased in the placentas from severe PE. In JEG-3 cells, hypoxia (O2 < 1%) induced a modest but not significant increase in the expression of LC3-II with a significant decrease in the expression of beclin-1. Meanwhile, TNF-{alpha} treatment induced a significant increase in the expression of LC3-II without a significant change in the expression of beclin-1. Our data suggests that increased autophagic activity in the placenta may be implicated in the pathophysiology of PE.

Key Words: Placenta • autophagy • preeclampsia • beclin-1 • LC3.

Reproductive Sciences, Vol. 15, No. 9, 912-920 (2008)
DOI: 10.1177/1933719108319159
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