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Reproductive Sciences
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Influence of Extracellular Matrix on Cytokine Stimulated Pro-labour Gene Expression in Human Uterine Myocytes

Neelam Engineer, MD

Imperial College Parturition Research Group, Department of Maternal Fetal Medicine, Imperial College School of Medicine, Chelsea & Westminster Hospital, London, nejengineer{at}aol.com

Suren R. Sooranna, BSc, PhD

Imperial College Parturition Research Group, Department of Maternal Fetal Medicine, Imperial College School of Medicine, Chelsea & Westminster Hospital, London

Zhiqing Liang, MD, PhD

Department of Obstetrics & Gynaecology, Southwest Hospital, Third Military Medical University, Chongqing, China

Phillip R. Bennett, MD, PhD

Imperial College Parturition Research Group, Institute of Reproductive and Developmental Biology, Hammersmith Hospital Campus, London

Mark R. Johnson, MD, PhD

Imperial College Parturition Research Group, Department of Maternal Fetal Medicine, Imperial College School of Medicine, Chelsea & Westminster Hospital, London

Cellular function is modulated by the interaction with the extracellular matrix within the myometrium. We formed the hypothesis that the cytokine-stimulated pro-labour gene expression by human uterine smooth muscle cells would be increased by growing the cells on collagen-coated plates. Primary cultures of human uterine smooth muscle cells grown on uncoated plates and on plates coated with collagen were exposed to the inflammatory cytokines (tumor necrosis factor—alpha, interleukin-1beta and interleukin-6) and assessed the messenger RNA expression of oxytocin receptor, interleukin-8, prostaglandin H synthase type-2 and prostaglandin F2 alpha receptor. Basal pro-labour gene expression was unaffected by collagen coating and the response to the inflammatory cytokines was similar for oxytocin receptor and prostaglandin H synthase type-2, but appeared to be reduced for interleukin-8 and enhanced for FP. Collagen coating made no significant impact on basal integrin expression and interleukin-1beta induced phosphorylation of extracellular-regulated-kinase1/2 and RelA subunit of nuclear factor—kappa B (p65). We conclude that growing human uterine smooth muscle cells on collagen-coated plates may modulate the pro-labour gene response to the inflammatory cytokines.

Key Words: Collagen • integrins • pro-labour genes • cytokines • FP receptor • myometrium.

Reproductive Sciences, Vol. 15, No. 9, 950-960 (2008)
DOI: 10.1177/1933719108322439
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