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Reproductive Sciences
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Role of Protein Kinase C{alpha} in Regulation of [Ca2+]I and Force in Human Myometrium

Victor P. Fomin, PhD

Department of Biological Sciences, University of Delaware, Newark, Delaware, vfomin{at}udel.edu

Andris Kronbergs, MS

Department of Biological Sciences, University of Delaware, Newark, Delaware

Susan Gunst, PhD

Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, Indiana

Dale Tang, PhD

Center for Cardiovascular Sciences, Albany Medical College, Albany, New York

Vladimir Simirskii, PhD

Department of Biological Sciences, University of Delaware, Newark, Delaware

Matthew Hoffman, MD

Department of Obstetrics and Gynecology, Christiana Hospital, Newark, Delaware

Randall L. Duncan, PhD

Department of Biological Sciences, University of Delaware, Newark, Delaware

Recent findings implicate protein kinase C in regulation of contraction of uterine muscle (myometrium). However, the role of protein kinase C isoforms in myometrial contraction remains uncertain. Therefore, this study examined protein kinase C{alpha}'s role in regulation of contraction and intracellular calcium concentration ([Ca2+]I) of myometrium from term pregnant women. The authors demonstrated that protein kinase C{alpha} inhibitor Go6976 decreased the amplitude of potassium chloride—induced myometrial contractions in a time-dependent manner. The treatment of the myometrial strips with protein kinase C{alpha}—specific antisense oligodeoxynucleotides decreased the potassium chloride—induced contraction and [Ca2+]I response to 39.3% + 6.8% and 50.0% + 3.3%, respectively, compared to control. The sense oligonucleotides treatment did not significantly change the potassium chloride responses (89.8% + 6.8% and 93.9% + 4.5% of the control for the contraction and [Ca2+]I, respectively). These data, coupled with the observation that protein kinase C{alpha} levels are elevated in the pregnant myometrium, suggest the involvement of protein kinase C{alpha} in regulation of human uterine contraction.

Key Words: Uterine muscle • human myometrium • intracellular calcium • protein kinase C • pregnancy.

This version was published on January 1, 2009

Reproductive Sciences, Vol. 16, No. 1, 71-79 (2009)
DOI: 10.1177/1933719108324892
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