Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Reproductive Sciences
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
1933719109339217v1
16/10/986    most recent
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Yeh, J.
Right arrow Articles by Page, C.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yeh, J.
Right arrow Articles by Beom Su Kim,
Right arrow Articles by Peresie, J.
Right arrow Articles by Page, C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Declines in Levels of Hyperpolarization-Activated Cation (HCN) Channels in the Rat Ovary After Cisplatin Exposure

John Yeh, MD

Department of Gynecology-Obstetrics, University at Buffalo, The State University of New York, Buffalo, New York, jyeh{at}buffalo.edu

Beom Su Kim, PhD

Department of Gynecology-Obstetrics, University at Buffalo, The State University of New York, Buffalo, New York

Jennifer Peresie, BS

Department of Gynecology-Obstetrics, University at Buffalo, The State University of New York, Buffalo, New York

Carly Page, BS

Department of Gynecology-Obstetrics, University at Buffalo, The State University of New York, Buffalo, New York

The distribution of the 4 hyperpolarization-activated cation (hyperpolarization-activated, cyclic nucleotide-gated [HCN]) channels (HCN1-4), channels associated with rhythmic electrical bursting, varies in the different cells of the ovary. Cisplatin is a broadly used chemotherapeutic agent that results in ovarian damage. The objective of this study was to test the hypothesis that cisplatin treatment will affect expression of the ovarian HCN channels. Adult female Sprague-Dawley rats were dosed with saline, 4.5 mg/kg cisplatin, or 6.0 mg/kg cisplatin as 2 weekly injections and then were killed 5 days after the second cisplatin dose. The ovaries were studied for HCN1-4 using semiquantitative H score immunohistochemical analysis and using Western blot analysis. By immunohistochemistry, for HCN4, the oocyte-specific staining declined after cisplatin treatment (P < .001). There was a decline in HCN2 intensity after cisplatin in the antral granulosa cells (P < .05) and thecal cells (P < .05). There were no differences found for the immunohistochemical HCN1 and HCN3 H scores after cisplatin. The Western blot analysis revealed that there was a decline in HCN3 levels after cisplatin exposure (P < .05). For HCN1 and HCN2, there was no change in the total ovarian protein levels after cisplatin. Treatment in vivo with cisplatin resulted in decline in the levels of the HCN channels in the rat ovary, with oocytes, antral cells, and thecal cells being specifically affected.

Key Words: Ovary • oocytes • HCN channels • cisplatin • chemotherapy.

This version was published on October 1, 2009

Reproductive Sciences, Vol. 16, No. 10, 986-994 (2009)
DOI: 10.1177/1933719109339217
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?