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Plasma From Women With Preeclampsia Inhibits Trophoblast Invasion

Maternal and Fetal Health Research Group, University of Manchester, St. Mary's Hospital, Oxford Road, Manchester, M13 9WL, United Kingdom, lynda.k.harris{at}manchester.ac.uk

O.H. Clancy, MD

Maternal and Fetal Health Research Group, University of Manchester, St. Mary's Hospital, Oxford Road, Manchester, M13 9WL, United Kingdom

J.E. Myers, MD, PhD

Maternal and Fetal Health Research Group, University of Manchester, St. Mary's Hospital, Oxford Road, Manchester, M13 9WL, United Kingdom

P.N. Baker, MD

Maternal and Fetal Health Research Group, University of Manchester, St. Mary's Hospital, Oxford Road, Manchester, M13 9WL, United Kingdom

To assess whether plasma from women with preeclampsia altered trophoblast invasion, SGHPL-4 extravillous trophoblasts were treated with pooled plasma from women with preeclampsia (PE-P; 10%) or with plasma from healthy pregnant controls (C-P). PE-P significantly inhibited SGHPL-4 invasion through Matrigel-coated transwells (P < .01), reduced mitochondrial dehydrogenase activity (P < .01), and increased apoptosis (P < .05); however, invading cells were no more susceptible to PE-P-induced apoptosis than their static counterparts. C-P did not alter rates of invasion, proliferation, or apoptosis. The bioactivity of PE-P was retained after removal of the 6 most abundant plasma proteins using an immunodepletion column (P < .05). Fractionation of PE-P demonstrated that the reduction in invasion was predominantly mediated by factors >100 kd in size. The authors conclude that plasma from women with preeclampsia contains multiple factors that inhibit invasion. These factors do not specifically target invading cells, but instead may reduce the number of cells available to invade.

Key Words: Apoptosis • plasma • preeclampsia • proliferation • trophoblast invasion.

This version was published on November 1, 2009

Reproductive Sciences, Vol. 16, No. 11, 1082-1090 (2009)
DOI: 10.1177/1933719109341842


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