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Reproductive Sciences
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Recombinant Epidermal Growth Factor Maintains Follicular Ultrastructure and Promotes the Transition to Primary Follicles in Caprine Ovarian Tissue Cultured In Vitro

J.J.H. Celestino, M.S.

Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE Brazil, juliana_jales{at}yahoo.com.br

J.B. Bruno, M.S.

Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE Brazil

I.B. Lima-Verde, M.S.

Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE Brazil

M.H.T. Matos, PhD

Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE Brazil

M.V.A. Saraiva, M.S.

Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE Brazil

R.N. Chaves, M.S.

Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE Brazil

F.S. Martins, M.S.

Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE Brazil

L.F. Lima

Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE Brazil

K.P.O. Name, M.S.

Department of Cell Biology, Laboratory of Electron Microscopy, University of Brasilia, Brasilia-DF, Brazil

C.C. Campello, PhD

Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE Brazil

J.R.V. Silva, PhD

Biotechnology Nucleus of Sobral (NUBIS), Federal University of Ceara, Sobral-CE Brazil

S.N. Bao, PhD

Department of Cell Biology, Laboratory of Electron Microscopy, University of Brasilia, Brasilia-DF, Brazil

J.R. Figueiredo, PhD

Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE Brazil

We investigated the effects of epidermal growth factor on the survival and growth of caprine preantral follicles. Ovarian fragments were cultured for 1 and 7 days in enriched minimal essential medium with epidermal growth factor (0, 1, 10, 50, 100, or 200 ng/mL). Non-cultured and cultured tissues were processed for histological and ultrastructural studies. Results showed that after 7 days, the epidermal growth factor (1 and 10 ng/mL) maintained the percentage of normal follicles similar to control. An increase in the percentage of primary follicles was observed with 1, 10, and 50 ng/mL of epidermal growth factor compared to enriched minimal essential medium. Ultrastructural studies confirmed follicular integrity after 7 days in epidermal growth factor (1 and 10 ng/mL). In conclusion, the low concentrations of epidermal growth factor maintain caprine follicular viability and promote the transition from primordial to primary follicles.

Key Words: EGF • preantral follicles • activation • ruminant.

This version was published on March 1, 2009

Reproductive Sciences, Vol. 16, No. 3, 239-246 (2009)
DOI: 10.1177/1933719108325756
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