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Reproductive Sciences
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Plasma From Preeclamptic Women Stimulates Transendothelial Migration of Neutrophils

Scott W. Walsh, PhD

Departments of Obstetrics and Gynecology, and Physiology, Virginia Commonwealth University Medical Center, Richmond, Virginia, swwalsh{at}vcu.edu

Neutrophils infiltrate systemic vasculature of women with preeclampsia, so we tested the hypothesis that factors in plasma of preeclamptic women activate endothelial cells to produce IL-8 resulting in transendothelial migration of neutrophils. Neutrophil migration was studied using the Transwell system. An endothelial cell line was grown to confluence on the inserts and treated with 10% plasma from normal nonpregnant (NNP), normal pregnant (NP) and preeclamptic (PE) women or with an oxidizing solution containing linoleic acid (OxLA). Compared to medium control, NNP plasma or NP plasma, PE plasma significantly stimulated IL-8 and neutrophil migration which was inhibited by vitamins E and C or IL-8 neutralizing antibody. Compared to medium control or LA, OxLA stimulated IL-8 and neutrophil migration which was inhibited by vitamins E and C or IL-8 antibody. Conclusion: Factors present in plasma of preeclamptic women stimulate transendothelial migration of neutrophils which is due to induction of oxidative stress and production of IL-8.

Key Words: Preeclampsia • neutrophils • IL-8 • oxidative stress • endothelial cells.

This version was published on March 1, 2009

Reproductive Sciences, Vol. 16, No. 3, 320-325 (2009)
DOI: 10.1177/1933719108327594
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L.K. Harris, O.H. Clancy, J.E. Myers, and P.N. Baker
Plasma From Women With Preeclampsia Inhibits Trophoblast Invasion
Reproductive Sciences, November 1, 2009; 16(11): 1082 - 1090.
[Abstract] [PDF]