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PP13 mRNA Expression in Trophoblasts From Preeclamptic Placentas

Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan, sekizawa{at}med.showa-u.ac.jp

Yuditiya Purwosunu, MD

Department of Obstetrics and Gynecology, University of Indonesia, Indonesia

Shiho Yoshimura, MD

Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan

Masamitsu Nakamura, MD

Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan

Hanako Shimizu, MD

Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan

Takashi Okai, MD

Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan

Nicola Rizzo, MD

Division of Prenatal Medicine Department of Histology and Embryology, University of Bologna, Bologna, Italy

Antonio Farina, MD

Division of Prenatal Medicine Department of Histology and Embryology, University of Bologna, Bologna, Italy

To assess the role of placental protein 13 (PP13) in the pathogenesis of preeclampsia, the messenger RNA expression of PP13 was investigated in the trophoblasts from first and third trimester placenta. First, syncytiotrophoblasts and extravillous trophoblasts were separated from frozen section of third trimester placenta obtained from pregnant women with and without preeclampsia by laser microdissection. The PP13 levels of syncytiotrophoblasts were significantly higher than those of extravillous trophoblasts. The expression levels in syncytiotrophoblasts from the preeclampsia were significantly lower than those from normal. Next, the PP13 expression was assessed in trophoblasts from residual samples of chorionic villus sampling at 11 weeks of gestation, who subsequently developed preeclampsia. The levels in the preeclampsia group were significantly lower than those of normal cases. These findings indicate that an alteration in the PP13-messenger RNA expression in the trophoblasts may be associated with the pathogenesis of preeclampsia.

Key Words: Trophoblast • PP13 • mRNA expression • preeclampsia.

This version was published on April 1, 2009

Reproductive Sciences, Vol. 16, No. 4, 408-413 (2009)
DOI: 10.1177/1933719108328615


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