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Elevated Plasma Stromal Cell-derived Factor 1 Protein and its Gene Polymorphism in Patients With Pelvic Inflammatory Disease

School of Nursing, College of Nursing, Chung Shan Medical University, Taichung, Taiwan

Yi-Torng Tee, MD, PhD

Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung, Taiwan, School of Medicine, Chung Shan Medical University, Taichung, Taiwan

Yi-Hsien Hsieh, PhD

Center for Molecular Medicine, China Medical University Hospital, Taichung, Taiwan

Hui-Ling Chiou, PhD

School of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung, Taiwan, Department of Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan

Chiao-Wen Lin, MS

Department of Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan

Hsiu-Chen Tsai, PhD

School of Nursing, College of Nursing, Chung Shan Medical University, Taichung, Taiwan

Po-Hui Wang, MD, PhD

Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung, Taiwan, School of Medicine, Chung Shan Medical University, Taichung, Taiwan

Shun-Fa Yang, PhD

Department of Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan, Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan, Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan, ysf{at}csmu.edu.tw

The objective was to compare the expression of plasma stromal cell-derived factor 1 and the gene polymorphism in patients with pelvic inflammatory disease and healthy controls. The enzyme-linked immunosorbent assay and polymerase chain reaction—restriction fragment length polymorphism were, respectively, used to measure the plasma stromal cell-derived factor 1 level and stromal cell-derived factor 1 polymorphism in 50 healthy controls and in 44 patients with pelvic inflammatory disease before and after they received routine treatment protocols. The level of plasma stromal cell-derived factor 1 was elevated in patients with pelvic inflammatory disease compared to normal controls and decreased significantly after treatment. There were significant correlations between plasma stromal cell-derived factor 1 level and neutrophil count as well as between stromal cell-derived factor 1 level and white blood cell count in patients with pelvic inflammatory disease. There was no significantly different distribution of stromal cell-derived factor 1 genotypes between patients with pelvic inflammatory disease and normal controls. Patients with pelvic inflammatory disease having stromal cell-derived factor 1-3'A allele were associated with significantly elevated plasma stromal cell-derived factor 1 concentration compared to patients with pelvic inflammatory disease having G/G homozygous alleles (P < .02). In normal controls, there was no significant difference in the plasma stromal cell-derived factor 1 level between individuals with and without stromal cell-derived factor 1-3'A allele. When the cutoff level of plasma stromal cell-derived factor 1 level was determined to be 2192 pg/mL based on receiver-operating characteristic curve, the sensitivity, specificity, positive predictive value, and negative predictive value as well as accuracy were 77.3%, 88.0%, 85.0%, 81.5%, and 83.0%. In conclusion, when the cutoff level was determined to be 2192 pg/mL, plasma stromal cell-derived factor 1 level can be used to predict pelvic inflammatory disease.

Key Words: Stromal cell-derived factor 1 (SDF-1) • single-nucleotide polymorphism (SNP) • pelvic inflammatory disease (PID).

This version was published on June 1, 2009

Reproductive Sciences, Vol. 16, No. 6, 610-617 (2009)
DOI: 10.1177/1933719109332829


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