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Reproductive Sciences
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Novel Alpha-Fetoprotein-V Messenger RNA Isoforms in Humans

Hikaru Tagaya, MD

Department of Obstetrics and Gynecology University of Yamanashi, Shimokato, Tamaho, Nakakoma, Yamanashi, Japan

Hiroko Fukasawa, MD, PhD

Department of Obstetrics and Gynecology University of Yamanashi, Shimokato, Tamaho, Nakakoma, Yamanashi, Japan

Tomoko Shoda, MD, PhD

Department of Obstetrics and Gynecology University of Yamanashi, Shimokato, Tamaho, Nakakoma, Yamanashi, Japan

Kazuhiko Hoshi, MD, PhD

Department of Obstetrics and Gynecology University of Yamanashi, Shimokato, Tamaho, Nakakoma, Yamanashi, Japan

Shuji Hirata, MD, PhD

Department of Obstetrics and Gynecology University of Yamanashi, Shimokato, Tamaho, Nakakoma, Yamanashi, Japan, shirata{at}yamanashi.ac.jp

It has been demonstrated that several messenger RNA (mRNA) isoforms have been transcribed from the alpha-fetoprotein (AFP) gene. In rats, it was reported that the novel exon, termed the exon V, exists between the exons 7 and 8, and the novel mRNA isoform (termed AFP-V mRNA) is synthesized using the exon V. In this study, a reverse transcription-polymerase chain reaction was performed and quantitative analysis was done on the AFP mRNA to identify the exon V and the AFP-V mRNA in humans. As a result, 2 novel exons, the exons VA and VB, were identified. Furthermore, 3 novel AFP mRNAs, the AFP-V1, -V2, and -V3 mRNA, were demonstrated to be expressed through alternative splicing. Expression of the AFP-V2 mRNA isoform and the wild-type mRNA was differentially regulated, implying that the AFP-V mRNA isoforms could be used in diagnosis and classification of hepatocellular carcinoma and ovarian carcinoma.

Key Words: Alpha-fetoprotein (AFP) mRNA • AFP-V mRNA isoform • exon V • human.

This version was published on August 1, 2009

Reproductive Sciences, Vol. 16, No. 8, 794-801 (2009)
DOI: 10.1177/1933719109336617
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