This Article Full Text (PDF) All Versions of this Article: 1933719109336621v1 16/9/843    most recent References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Scopus Google Scholar Articles by Youssef, R. E. Articles by Norman, J. E. PubMed PubMed Citation Articles by Youssef, R. E. Articles by Ledingham, M. A. Articles by Bollapragada, S. S. Articles by O'Gorman, N. Articles by Jordan, F. Articles by Young, A. Articles by Norman, J. E. Social Bookmarking                         What's this?

The Role of Toll-Like Receptors (TLR-2 and -4) and Triggering Receptor Expressed on Myeloid Cells 1 (TREM-1) in Human Term and Preterm Labor

Ninewells Hospital and School of Medicine, Dundee, United Kingdom, ryoussef{at}nhs.net

Marie Anne Ledingham, MD, MRCOG

The Queen Mother's Hospital, Yorkhill, Glasgow, United Kingdom

Shrikant S. Bollapragada, MRCOG

Reproductive & Maternal Medicine, University of Glasgow, Third Floor, Queen Elizabeth Building, Glasgow Royal Infirmary, United Kingdom

Neil O'Gorman, MB, CHB

Reproductive & Maternal Medicine, University of Glasgow, Third Floor, Queen Elizabeth Building, Glasgow Royal Infirmary, United Kingdom

Fiona Jordan, MSc

Reproductive & Maternal Medicine, University of Glasgow, Third Floor, Queen Elizabeth Building, Glasgow Royal Infirmary, United Kingdom

Anne Young, MSc

Reproductive & Maternal Medicine, University of Glasgow, Third Floor, Queen Elizabeth Building, Glasgow Royal Infirmary, United Kingdom

Jane E. Norman, MD, FRCOG

University of Edinburgh Centre for Reproductive Biology, The Queen's Medical Research Institute, Edinburgh, United Kingdom

Objective. To define the role of Toll-like receptors (TLR-2, TLR-4) and triggering receptor expressed on myeloid cells-1 (TREM-1) in pregnant human myometrium. Study Design. Expression of TLR-2 and -4 mRNA and protein and TREM-1 mRNA was quantified in human myometrial samples. Subsequently, we investigated the effect of medroxyprogesterone acetate (MPA) as a functional inhibitor of the TLR agonist lipopolysaccharide (LPS). Results. Messenger RNA expression of TLR-2 and -4 was significantly higher in myometrium at term compared with preterm (P = .009 and .016, respectively). Toll-like receptor-2 protein expression was significantly higher during labor (P = .002) compared with nonlaboring samples. Triggering receptor expressed on myeloid cells-1 mRNA expression was increased in both myometrium and cervix after labor at term (P < .05). Medroxyprogesterone acetate significantly suppressed LPS-induced production of interleukin 1b (IL-1b), IL-6, and IL-8 in vitro (P < .05). Conclusion. Toll-like receptors 2and 4 and TREM-1 are expressed in human myometrium and may play a role in the mechanism of labor at term, and their functional effects are inhibited by MPA.

Key Words: Inflammation • mechanism of human parturition.

This version was published on September 1, 2009

Reproductive Sciences, Vol. 16, No. 9, 843-856 (2009)
DOI: 10.1177/1933719109336621


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?