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Proteomic Analysis of the Luteal Endometrial Secretome

Division of Reproductive Endocrinology and Infertility, Department of Obstetrics & Gynecology, University of North Carolina, Chapel Hill, North Carolina

Marc A. Fritz, MD

Division of Reproductive Endocrinology and Infertility, Department of Obstetrics & Gynecology, University of North Carolina, Chapel Hill, North Carolina

Mihaela Mocanu, MS

UNC-Duke Michael Hooker Proteomics Center, University of North Carolina, Chapel Hill, North Carolina

Bruce A. Lessey, MD, PhD

Reproductive Endocrinology and Infertility, Department of Obstetrics & Gynecology, Greenville Hospital System, Greenville, South Carolina

Steven L. Young, MD, PhD

Division of Reproductive Endocrinology and Infertility, Department of Obstetrics & Gynecology, University of North Carolina, Chapel Hill, North Carolina, youngs{at}med.unc.edu

Endometrium attains a secretory architecture in preparation for embryo implantation, but the identity of most endometrial secretory products remains unknown. Our objective was to characterize the endometrial secretome and compare protein expression between prereceptive (luteinizing hormone [LH]+4) and receptive (LH+9) phase endometrium. Endometrial lavage was performed in 11 participants and analyzed by difference gel electrophoresis (DIGE). LH+4 and LH+9 specimens were labeled with cyanine fluorescent dyes Cy3 and Cy5 tags, respectively, and combined. Proteins were separated using 2-dimensional gel electrophoresis, isolated, trypsin-digested, and subjected to mass spectrometry. In all, 152 proteins were identified; 82 were differentially expressed. Most proteins with increased expression on LH+9 functioned in host defense, while proteins with decreased expression had many functions. A total of 14 proteins had changes suggesting altered posttranslational modification. This article describes the first application of proteomic analysis to endometrial secretions, allowing identification of novel endometrial proteins as well as those differentially secreted in prereceptive and receptive phases.

Key Words: Proteomics • endometrium • luteal phase • secretome • endometrial receptivity • fibrinogen gamma.

This version was published on September 1, 2009

Reproductive Sciences, Vol. 16, No. 9, 883-893 (2009)
DOI: 10.1177/1933719109337165


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