Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of the Society for Gynecologic Investigation
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Gorodeski, G. I.
Right arrow Articles by Hopfer, U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gorodeski, G. I.
Right arrow Articles by Hopfer, U.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Regulation of the Paracellular Permeability of Cultured Human Cervical Epithelium by a Nucleotide Receptor

George I. Gorodeski, MD, PhD

Ulrich Hopfer, MD, PhD

Departments of Reproductive Biology and Physiology and Biophysics, Case Western Reserve School of Medicine; Department of Obstetrics and Gynecology, University MacDonald Womens Hospital, Cleveland, Ohio

Objective: We sought to determine what type of regulation of transepithelial transport in leaky epithelium can occur at the level of paracellular permeability.

Methods: The epithelial permeability to the polar acid pyramine (Ppyr) and the transepithelial electrical resistance (R) were determined in cultured human cervical epithelium.

Results: Extracellular adenosine triphosphate (ATP) acutely and reversibly decreased the paracellular permeability, as evidenced by an increase of R from 10 ± 3 to 16 ± 2 µ/cm2 and a decrease in Ppyr from 13 ± 3 to 9 ± 3 x 10-6 cm/sec and (P < .01 for both). The ATP effect was dose related (average median effective concentration 2 ± 1 µM), saturable at 50 µM, and desensitized with repeated administration; it was mimicked by uridine triphosphate and ATP-{gamma}-S but not by adenosine monophosphate, ademine, adenosine, or adenosine diphosphate. The ATP effect on resistance remained intact even when the intercellular resistance was decreased with a basal-to-apical pressure gradient, but was abolished by lowering extracellular calcium.

Conclusions: These results indicate the following: 1) Paracellular permeability in the cervical epithelial cell line is regulated by a nucleotide receptor; and 2) the tight junctions are effectors of nucleotide-receptor stimulation. We suggest that extracellular ATP may regulate mucus production in the cervix in vivo by acting on a surface receptor and by increasing the resistance of the tight junctions.

Key Words: Paracellular permeability • paracellular transport • extracellular ATP • nucleotide receptors

Journal of the Society for Gynecologic Investigation, Vol. 2, No. 5, 716-720 (1995)
DOI: 10.1177/107155769500200508
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?