Journal of the Society for Gynecologic Investigation

 

Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

http://mc.manuscriptcentral.com/rsci

SAGETRACK

Sign In to gain access to subscriptions and/or personal tools.
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dudley, D. J.
Right arrow Articles by Mitchell, M. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of the Society for Gynecologic Investigation, Vol. 3, No. 1, 12-16 (1996)
DOI: 10.1177/107155769600300103

Macrophage Inflammatory Protein-1{alpha} Regulates Prostaglandin E2 and Interleukin-6 Production by Human Gestational Tissues In Vitro

Donald J. Dudley, MD

S. S. Edwin, BS

Murray D. Mitchell, DPhil, DSc

Department of Obstetrics and Gynecology, University of Utah School of Medicine, Salt Lake City, Utah

Objective: To determine if the inflammatory cytokine macrophage inflammatory protein-l{alpha} (MIP-1{alpha}) stimulates the production of prostaglandin E2 (PGE2) and interleukin-6 (IL-6) by human gestational tissues.

Methods: Human decidual cells. chorion cells, and amnion cells were isolated from term placentas of women with normal pregnancies, and primary cultures of each cell line were established. In various concentrations, MIP-1{alpha} was then applied to each cell culture for 16 hours at 37C, and culture supernatants were assayed for PGE2 and IL-6.

Results: After incubation with MIP-1{alpha}, decidual cells produced increased amounts of IL-6 (P < .05) but not PGE2. When subjected to similar conditions, chorion cells produced increased amounts of both IL-6 and PGE2 (P < .05). Conversely, amnion cells produced increased amounts of only PGE2 in response to increasing concentrations of MIP-1{alpha} (P < .05).

Conclusion: Maternal and fetal gestational tissues differentially respond to MIP-1{alpha} with regard to PGE2 and IL-6 production; MIP-1{alpha} may play a role in both the initiation and propagation of the inflammatory response associated with intrauterine infection.

Key Words: Macrophage inflammatory protein-1{alpha} • prostaglandin E2 • interleukin-6


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?