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Detection and In Vivo Hormonal Regulation of Rat Ovarian Type I and Type II Interleukin-I Rececptor mRNAs: Increased Expression During the Periovulatory Period

Kristiina S. L. Ruutiainen-Altman, MD

Lechoslaw T. Putowski, MD, PhD

Shahar Kol, MD

Eli Y. Adashi, MD

Richard M. Rohan, PhD

Division of Reproductive Endocrinology, Department of Obsterics and Gynecology, University of Maryland School of Medicine, Baltimore, Maryland

Objective: To study the expression, localization, and in vivo hormonal regulation of type I and type II interleukin-1 (IL-1) receptors in the rat ovary.

Methods: Segments of the cDNAs for rat type I and type II IL-1 receptors were cloned and used as probes in RNase protection assays and in situ hybridization. Tissues obtained from immature rats and hormonally treated rat ovaries were examined.

Results: Type I IL-1 receptor (IL-1R(1)) was ubiquitously expressed in rat tissues, including, granulosa cells prepared from immature ovaries, whereas type II IL-1 recetor (IL-1R(2)) expression was restricted to macrophage, thymus, and lung. Hypophysectomy and subsequent treatment with FSH and/or diethylstilbestrol did not alter significantly the abundance of IL-1R(1) transcripts in the whole overy. However, the relative amount of ovarian IL-1R(1) transcripts increased 7.3-fold 6 hours after the administration of hCG to pregnant mare serun gonadotropin—primed immature rats. During this time, IL-1R(1) mRNA was localized primarily in the granulosa cells. The increased expression of IL-1R(1) persisted 24 hours after hCG administration but declined to baseline by 48 hours. Ovarian expression of IL-1R(2) mRNA was observed only before ovulation in amounts that were approximately 70-fold lower than IL-1R(1).

Donclusion: The increased intraovarian expression of IL-1R(1) in granulosa cells during the periovulatory period implies that this cell type has a heightened receptivity to IL-1 and provides further indirect evidence that this cytokine is involved in the ovulatory process.

Key Words: Receptors • interleukin-1 • Gene expression • ovary

Journal of the Society for Gynecologic Investigation, Vol. 3, No. 3, 131-139 (1996)
DOI: 10.1177/107155769600300306


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