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Journal of the Society for Gynecologic Investigation, Vol. 3, No. 4, 172-178 (1996)
DOI: 10.1177/107155769600300403
© 1996 SAGE Publications

Interleukin-1ß and Tumor Necrosis Factor-{alpha} Stimulate Granulocyte Colony-Stimulating Factor Production by Placental Villous Core Mesenchymal Cells

David T. Vandermolen, MD

Scott W. Kauma, MD

Tom T. Turner, BS

Department of Obstetrics and Gynecology, Medical College of Virgima, Richmond, Virginia

Objective: To test the hypothesis that interleukin- 1ß (IL-ß) and tumor necrosis factor-{alpha} (TNF-{alpha}) regulate granulocyte colony-stimulating factor (G-CSF) production by by human placental villous core mesenchymal cells.

Methods: Villous core mesenchymal cells were isolated from placents at 14-20 weeks' gestation and cultured in vitro. Cells were treated with IL-1ß or TNF-{alpha} in dose-response and time-course studies. We measured G-CSF mRNA expression by Northem blot analysis and G-CSF protein production by enzyme-linked immunosorbent assay of the conditioned media.

Results: Unstimulated mesenchmal cells expressed negligible G-CSF, Steady-state G-CSF mRNA expression was maximal 3-6 hours after IL-1ß treatment and 6-18 hours after TNF-{alpha} treatment. Each cytokine induced G-CSF protein production in dose- and time-dependent manners, with IL-1ß more potent than TNF-{alpha}. The G-CSF mRNA expression and G-CSf production induced by the combination of both cytokines exceeded that induced by either cytokine alone.

Conclusions: Interleukin-1ß and TNF-{alpha} stimulate G-CSF production by placental villous core mesenchymal cells in vitro. These results identify a potential mechanism by which villous core mesenchymal cells mediate, in part, the response to these two cytokines.

Key Words: Granulocyte colony-stimulating factor • interleukin-1ß • placenta • tumor necrosis factor-{alpha}


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