Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to register

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of the Society for Gynecologic Investigation
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Miller, J. B.
Right arrow Articles by Obasiolu, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miller, J. B.
Right arrow Articles by Obasiolu, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

The Effect of Insulin and Insulin-Like Growth Factors on Luteal Progesterone Secretion in the Rabbit

Josephine B. Miller, PhD

University of Illinois at Chicago, Department of Obstetrics and Gynecology, M/C 808 820 S. Wood Street, Chicago, IL 60612-7313

Charles Obasiolu, MD

Department of Obstetrics and Gynecology, University of Illinois at Chicago, Chicago, Illinois

Objective: The purpose of this study was to determine if insulin or insulin-like growth factors (IGF) either alone or in combination with estradiol (E2) could maintain progesterone secretion by rabbit luteal cells in culture.

Methods: Progesterone secretion was measured in media of luteal cell cultures incubated in serum-free media alone or containing E2 with or without added insulin, IGF-I or IGF-II.

Results: Progesterone production by rabbit luteal cells in culture fell precipitously in serum-free media containing E2, IGF-I, IGF-II, or insulin alone. However, when E2 was combined with insulin, IGF-I, or IGF-II, serum progesterone production was stimulatewd two- to fivefold above control levels.

Conclusion: There is an important synergism between insulin/IGF and E2 in regulating luteal steroidogenesis.

Key Words: Rabbit luteal cell cultures • estradiol • insulin • IGF-I • IGF-II

Journal of the Society for Gynecologic Investigation, Vol. 3, No. 5, 262-266 (1996)
DOI: 10.1177/107155769600300506
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?