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Journal of the Society for Gynecologic Investigation
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The Transfer of Prostaglandin E2 Across Ovine Fetal Membranes In Vivo

Pauline C. Johnston, PhD

Ian A. Greer, MD

A. Nigel Brooks, PhD

Department of Obsterrics and Gynecology, University of Glasgow, Royal Infirmary, Glasgow; Medical Research Council Reproductive Biology Unit. Edinburgh, Scotland

Objective: This study investigated whether prostaglandin E2 (PGE2) can cross intact fetal membranes in vivo.

Methods: We have developed a novel ovine model using in vivo dialysis systems, positioned between the fetal membranes and decidua and in the amniotic cavity. Sheep were given 25 µCi, 50 ng [3H]PGE2 or 1 mg PGE2 to the amniotic cavity, and radioactivity or PGE2/PGEM concentrations in dialysates were determined.

Results: Endogenous basal PGE2 concentrations were significantly higher iun extra-amniotic than in intra-amniotic dialysates (715 ± 436 versus 80 ± 31 pg/mL; mean ± standard error of the mean; p < .05). After injection of [3]PGE2 into the amniotic cavity, no radioactivity was detected in the extraamniotic dialysate, and this was not influenced by labor induced by ACTH administration to the fetus. In contrast, intra-amniotic injection of 1 mg PGE2 resulted in marked transfer of PEG2 across the fetal membranes to the extra-amniotic dialysis system.

Conclusion: These results suggest that PGE2 can cross the ovine fetal membranes in vivo, escaping metabolism in the chorion.

Key Words: Prostaglandin E2 • fetal membranes • parturition • ovine model

Journal of the Society for Gynecologic Investigation, Vol. 3, No. 6, 303-308 (1996)
DOI: 10.1177/107155769600300602
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