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Journal of the Society for Gynecologic Investigation
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Developmental Changes in Renin-Containing Cells From the Ovine Fetal Kidney

Jinjuan Wang, MD

Departments of Physiology and Pharmacology and Obstetrics and Gynecology and the Perinatal Research Laboratories, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina; Perinatal Research Laboratories, Bowman Gray School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157

Frank M. Perez, PhD

James C. Rose, PhD

Departments of Physiology and Pharmacology and Obstetrics and Gynecology and the Perinatal Research Laboratories, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina

Objective: To determine if the increase in intrarenal renin content that occurs in late gestation in the fetal lamb results from an increase in the proportion of cells containing renin, an increase in the amount of renin per renin-containing cell, or both.

Methods: Kidney cells with a density of 1.062 g/mL were isolated from ovine fetuses at 100-104 days gestation (n = 6, immature) and at 131-139 days gestation (n = 6, mature). This fraction was enriched with renin-containing cells. Total renin (TR) and active renin (AR) were measured by their ability to generate angiotensin I (AI). Renin mRNA was measured by RNase protection assay. The presence of renin in cells and the capacity to release it were identified by immunocytochemistry and cell immunoblot analyses. Data were analyzed by analyses of variance.

Results: The TR and AR in isolated cells increased from 3.7 ± 0.3 and 2.5 ± 0.2 µg AI/µg protein · hr-1 in immature fetuses to 6.2 ± 0.7 and 5.0 ± 0.6 µg AI/µg protein · hr-1 in mature fetuses (P < .01). The percentage of immunopositive renin cells (PC) was greater in mature (40.6 ± 4.3) than in immature fetuses 915.2 ± 1.2) (P < .01). The TR/PC and AR/PC were also higher in mature (8.2 ± 0.6 and 6.6 ± 0.4 ng AI/h · pc-1 than in immature animals (6.5 ± 0.6 and 4.4 ± 0.4 ng AI/h · pc-1) (P < .01).

Conclusions: The data indicate that the increase in TR and AR occurring during development in fetal lamb kidney results from an increase in both the number of renin-containing cells and in the renin content per cell.

Key Words: Renin • ovine fetus • kidney • renin gene • renin containing cells

Journal of the Society for Gynecologic Investigation, Vol. 4, No. 4, 191-196 (1997)
DOI: 10.1177/107155769700400404
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